| 冯云,张海林,付士红,杨卫红,章域震,王丕玉,杨捷,刘永华,董朝良,李轼,张保森,尹正留,董兴齐,王环宇,梁国栋.云南省德宏州2007年和2010年蚊虫及蚊媒病毒调查[J].中华流行病学杂志,2014,35(5):528-532 |
| 云南省德宏州2007年和2010年蚊虫及蚊媒病毒调查 |
| Investigation on mosquitoes and mosquito-borne viruses in Dehong prefecture, Yunnan province, 2007 and 2010 |
| 收稿日期:2013-09-23 出版日期:2014-09-17 |
| DOI：10.3760/cma.j.issn.0254-6450.2014.05.013 |
| 中文关键词: 蚊虫 流行性乙型脑炎病毒 盖塔病毒 淡色库蚊浓核病毒 分子生物学特征 |
| 英文关键词: Mosquito Japanese encephalitis virus Getah virus Culex pipiens pallens densovirus Molecular biological characteristics |
| 基金项目:中美新发和再发传染病合作项目(U19-GH000004)；国家自然科学基金(30560142) |
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| 中文摘要: |
| 目的 调查云南省德宏州蚊虫和蚊媒病毒分布特点。方法 2007年和2010年的7月在德宏州5个县市利用诱蚊灯采集蚊虫标本，采用C6/36和BHK-21细胞分离病毒，RT-PCR和序列分析方法鉴定病毒分离物。结果 共采集到6属29种43 634只蚊虫，其中三带喙库蚊构成比高达78.69%，中华按蚊构成比为14.77%，其他蚊种数量较少。从蚊虫中分离到6株病毒，经RT-PCR和进化分析表明，其中3株为基因Ⅰ型流行性乙型脑炎病毒 （JEV），均分离自三带喙库蚊；1株盖塔病毒 （GETV） 分离自三带喙库蚊； 2株淡色库蚊浓核病毒 （CppDNV） 分别分离自三带喙库蚊和迷糊按蚊。结论 三带喙库蚊是德宏州优势蚊种和蚊媒病毒主要传播媒介，当地存在基因Ⅰ型JEV、 GETV和CppDNV的流行，并与以往云南省及我国其他地区的分离株有较近亲缘关系。 |
| 英文摘要: |
| Objective To investigate the distribution patterns of mosquitoes and mosquito-borne viruses in Dehong prefecture, Yunnan province, China. Methods Mosquito samples were collected using the mosquito traps from five counties of Dehong prefecture on July, 2007 and 2010. Mosquitoes were cell cultured for viral isolation, and positive isolates were identified using RT-PCR and sequence analysis. Results A total of 43 634 mosquitoes comprised of 29 species representing six genera were collected. Culex tritaeriorhynchus and Anopheles sinensis comprised 78.69% and 14.77% of the total. Six strains of viruses were isolated from the mosquito pools. RT-PCR and phylogenetic analysis revealed three strains from Cx. tritaeriorhynchus, identified as genotype ⅠJapanese encephalitis virus(JEV). One strain was identified from Cx. tritaeriorhynchus, as Getah virus(GETV). Two strains isolated from Cx. tritaeriorhynchus and Anopheles vagus were identified as Culex pipiens pallens Densovirus(CppDNV). Conclusion Cx. tritaeriorhynchus had been the major species of mosquitoes and mainly transmitting vector of mosquito-borne viruses in Dehong prefecture. GenotypeⅠJEV, GETV and CppDNV were the vectors causing transmission of mosquitoe-borne diseases in this area. Data from phylogenetic analysis showed that these newly discovered isolates seemed to have had close relationship with those viruses previously circulating in Yunnan and other provinces of China. |
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