| 邓永岳,张春阳,严延生,颜苹苹,吴守丽.福建省HIV-1 新近感染者流行毒株基因亚型及其流行特征分析[J].中华流行病学杂志,2014,35(6):714-719 |
| 福建省HIV-1 新近感染者流行毒株基因亚型及其流行特征分析 |
| Genetic subtype and epidemiological feature of HIV-1 circulating strains among recently infected patients in Fujian province |
| 收稿日期:2014-02-12 出版日期:2014-09-02 |
| DOI： |
| 中文关键词: 艾滋病病毒 新近感染者 亚型 |
| 英文关键词: HIV-1 New recent infections Subtype |
| 基金项目:福建省卫生厅青年科研基金（2012-1-12） |
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| 中文摘要: |
| 目的 了解福建省HIV-1 新近感染者流行毒株的亚型分布和流行特征。方法 随机抽取福建省2011－2012 年使用BED捕获酶免疫试验检出的104 例新近感染者血样标本，采用反转录巢式PCR扩增,gag、pol 和env 基因片段，并测定和分析核酸序列。结果 目标人群中存在B亚型和01_AE、07_BC、08_BC、55_01B 四种流行重组型，其中以01_AE 和07_BC 流行重组型为主，分别占45.68%和35.80%。基因亚型的流行特征分析显示，亚型分布中01_AE 所占比例下降，07_BC 所占比例上升;性传播是主要感染途径，其中同性传播比例明显上升。结论 福建省HIV-1 流行毒株亚型分布众多，随着时间推移，亚型构成和传播途径已发生改变，应加强对HIV-1感染的分子流行病学趋势动态监测。 |
| 英文摘要: |
| Objective In order to evaluate the distribution of genetic subtypes and epidemiological feature of HIV-1 circulating strains in Fujian province. Methods Blood samples and epidemiological data were collected from 104 newly infected patients who were distinguished by BED-CEIA methodology，during 2011-2012. Viral sequences（n＝81）of HIV-1 gag，env，and pol segments were amplified by nested PCR. Results Subtypes B and four Circulating Recombinant Forms，（CRF01_AE，CRF07_BC，CRF08_BC and CRF55_01B） were found in the samples， CRF01_AE（45.68%）and CRF07_BC（35.80%）were the two main HIV-1 strains in Fujian province. Compared with previous data，the proportion of CRF07_BC rose significantly while it gradually decreased in CRF01_AE. Heterosexual contact was still the principal transmission route in Fujian province，but the number of infection among men-who-have-sex-with- men grew rapidly. Conclusion Results from this study suggested that different subtypes of HIV-1 strain existed in Fujian province. The distribution of subtypes and the mode of transmission were changing with the progress of epidemic. Dynamic monitoring of the molecular epidemiology trends of HIV-1 infection should be enhanced. |
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