文章摘要
郑丹,邓伟,黄天壬,李曦亮,李召发.广西壮族自治区肝癌高发区HBV基因型、BCP/前C区突变与肝癌相关性的研究[J].中华流行病学杂志,2015,36(7):725-729
广西壮族自治区肝癌高发区HBV基因型、BCP/前C区突变与肝癌相关性的研究
Relationship between hepatitis B virus genotype, BCP/Pre-C region mutations and risk of hepatocellular carcinoma in Guangxi Zhuang Autonomous Region
投稿时间:2014-12-09  
DOI:10.3760/cma.j.issn.0254-6450.2015.07.013
中文关键词: 肝癌;乙型肝炎病毒;基因型;突变
英文关键词: Hepatocellular carcinoma;Hepatitis B virus;Genotype;Mutation
基金项目:国家自然科学基金(30960333,81260319); 广西壮族自治区高校科技项目(2013ZD016)
作者单位E-mail
郑丹 530021 南宁, 广西医科大学  
邓伟 附属肿瘤医院  
黄天壬 附属肿瘤医院 tianrenhuang@sina.com 
李曦亮 530021 南宁, 广西医科大学  
李召发 530021 南宁, 广西医科大学  
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中文摘要:
      目的 研究广西壮族自治区(广西)扶绥县肝癌高发区HBV基因型、基本核心启动子(BCP)/前C区突变与肝癌的关系。方法 采用病例对照方法收集53例肝癌患者和70例HBV健康携带者的血清,提取HBV DNA,用巢式PCR扩增 HBV S区、BCP/前C区,扩增产物纯化后测序,分析基因型、基因突变与肝癌发生的关系。结果 BCP区A1762T/G1764A及前C区T1858C在病例组中的突变率高于对照组,分别为94.3% vs. 75.7%(P=0.006)和50.9% vs. 31.4%(P=0.029);A1775G在对照组中的突变率高于病例组28.6% vs.13.2%(P=0.041)。多因素logistic回归分析显示,HBV A1762T/G1764A和T1858C突变是肝癌发生的危险因素,OR值分别为5.459(95%CI:1.397~21.332,P=0.015)和3.881(95%CI:1.462~10.305,P=0.006);A1775G突变是肝癌发生的保护因素,OR=0.192(95%CI:0.059~0.622,P=0.006)。结论 HBV C基因型是广西肝癌高发区的主要流行株,HBV BCP区A1762T/G1764A、A1775G、前C区T1858C 突变与HBV相关肝癌的发生有密切关系。
英文摘要:
      Objective To investigate the relationship between hepatitis B virus (HBV) genotype, the mutation in basic core promoter(BCP) region/pre-core(Pre-C) region and the incidence of hepatocellular carcinoma(HCC) in Fusui county of Guangxi Zhuang Autonomous Region(Guangxi), a area with high incidence of HCC. Methods In this case-control study, 53 HCC patients and 70 asymptomatic HBV carriers were enrolled. Blood samples were collected from them for serum separation and HBV DNA extraction. The DNA sequences of the S region and BCP/Pre-C region of HBV was determined by direct sequencing following nested-PCR amplification. The relationship between the genotype, gene mutation of HBV and the incidence of HCC was analyzed. Results The mutation rates of the A1762T/G1764A in the BCP region and the T1858C in the Pre-C region of HBV were significantly higher in HCC group than in control group (94.3% vs. 75.7%, P=0.006; 50.9% vs. 31.4%, P=0.029). The mutation rate of A1775G was significantly higher in control group (28.6%) than in HCC group (13.2%) (P=0.041). Multiple logistic regression analysis indicated that A1762T/G1764A and T1858C mutations are the risk factors for the development of HCC(OR=5.459, 95%CI:1.397- 21.332, P=0.015;OR=3.881, 95%CI:1.462-10.305, P=0.006). A1775G is the protective factor in the development of HCC (OR=0.192, 95%CI:0.059-0.622, P=0.006). Conclusion The present investigation showed that BCP A1762T/G1764A, A1775G and Pre-C T1858C mutations are correlated with the incidence of HCC in Fusui county of Guangxi.
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