| 崔大伟,李中杰,林洁,靳淼,杨先知,谢国良,郑书发,余斐,陈瑜.杭州地区2014-2015年急性胃肠炎患者感染诺如病毒的流行病学特征分析[J].中华流行病学杂志,2016,37(2):254-258 |
| 杭州地区2014-2015年急性胃肠炎患者感染诺如病毒的流行病学特征分析 |
| Epidemiologic characteristics of noroviruses isolated in outpatients with acute gastroenteritis in Hangzhou area, from 2014 to 2015 |
| 收稿日期:2015-08-17 出版日期:2016-02-18 |
| DOI：10.3760/cma.j.issn.0254-6450.2016.02.021 |
| 中文关键词: 诺如病毒 急性胃肠炎 聚合酶链反应 基因型 |
| 英文关键词: Noroviruses Acute gastroenteritis Polymerase chain reaction Genotype |
| 基金项目:国家科技重大专项(2012ZX10004-210) |
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| 中文摘要: |
| 目的 了解2014-2015年杭州地区急性胃肠炎患者中诺如病毒的流行病学特征。方法 收集1 109份急性胃肠炎患者的临床流行病学资料和粪便样本,采用一步法双重荧光RT-PCR对样本中诺如病毒(GⅠ、GⅡ型)进行初筛,然后随机选择诺如病毒阳性的样本核酸进行多重RT-PCR扩增,对产物进行测序分析。结果 1 109份的粪便样本中,诺如病毒检出率为26.87%(298/1 109),以GⅡ亚型为主,占25.52%(283/1 109),GⅠ亚型占1.35%(15/1 109);不同性别人群诺如病毒检出率差异无统计学意义(P>0.05);不同年龄组人群中诺如病毒检出率分别为16.94%(<5岁)、19.45%(5~18岁)和32.26%(≥18岁);全年均可检出诺如病毒感染,高发季节为12月至次年3月,阳性率为29.67%~37.08%。序列分析显示,GⅡ基因亚型中以GⅡ-4/Sydney株为主(46.43%,13/28),其次为GⅡ-4/2006b株;GⅠ基因亚型中以GⅠ-1亚型为主(55.56%,5/9)。结论 诺如病毒是导致2014-2015年杭州地区急性胃肠炎的主要病原之一,GⅡ基因亚型特别是GⅡ-4/Sydney变异株和GⅡ-4/2006b变异株是该地区流行的优势毒株。 |
| 英文摘要: |
| Objective To explore the epidemiologic characteristics of noroviruses isolated in patients with acute gastroenteritis in Hangzhou between March 2014 and April 2015.Methods Stool specimens and clinical data were collected from 1 109 patients with acute gastroenteritis.Specimens were detected for noroviruses with GⅠ and GⅡ subtypes by one-step double real-time RT-PCR.Some of the positive specimens were then randomly selected and amplified by multiplex RT-PCR.Those positive PCR products were sequenced and analyzed phylogenetically for testing the partial capsids of noroviruses.Results Of the 1 109 stool specimens, positive rate of noroviruses was 26.87%(298/1 109).GⅡ genotype was the major viruses with the proportion as 25.52%(283/1 109), while 1.35%(15/1 109) belonged to GⅠ genotypes.There was no significant difference in the noroviruses detection rate of the different genders(P>0.05).However, in different age groups, GⅡ genotypes were predominant types of noroviruses, and the positive rates of GⅡ genotypes were 16.94%(<5 years-old), 19.45%(5-18 years-old) and 32.26%(≥18 years-old), respectively.In different seasons, noroviruses could be detected all year round, with positive rate as 29.67%-37.08% in the highly epidemic seasons(between December and March of the following year).The distribution trends were seen certain difference between noroviruses-GⅡ and GⅠ types.Additionally, Results from the sequence analysis demonstrated that GⅡ-4 genotype was the prevalent strain of GⅡ genotypes, clustered into GⅡ-4/Sydney(46.43%, 13/28) and GⅡ-4/2006b(25.0%, 7/28), while G Ⅰ strains clustered into GⅠ-1.Conclusion Noroviruses appeared one of the major pathogens, leading to acute gastroenteritis.GⅡ genotypes of noroviruses, especially the GⅡ-4/Sydney variant strains and GⅡ-4/2006b variant strains, were considered to be the prevalent strains prevailed in Hangzhou areas from 2014 to 2015. |
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