文章摘要
赵孝梅,何燕,张婷,王婵娟,张旺德,禹文峰,官志忠.HLA-DP基因2个单核苷酸多态性位点与贵州少数民族人群HBV感染的关联研究[J].中华流行病学杂志,2016,37(3):389-393
HLA-DP基因2个单核苷酸多态性位点与贵州少数民族人群HBV感染的关联研究
Study on association between HLA-DP gene polymorphism and susceptibility to hepatitis B virus infection in minority population in Guizhou province
收稿日期:2015-08-03  出版日期:2016-03-15
DOI:10.3760/cma.j.issn.0254-6450.2016.03.020
中文关键词: 肝炎病毒,乙型;人类白细胞抗原;单核苷酸多态性;少数民族
英文关键词: Hepatitis B virus;Human leukocyte antigen;Single nucleotide polymorphism;Minority population
基金项目:国家科技支撑计划(2013BAI05B03)
作者单位E-mail
赵孝梅 550004 贵阳, 贵州医科大学分子生物学重点实验室  
何燕 550004 贵阳, 贵州医科大学分子生物学重点实验室 annieheyan@gmc.edu.cn 
张婷 550004 贵阳, 贵州医科大学分子生物学重点实验室  
王婵娟 550004 贵阳, 贵州医科大学分子生物学重点实验室  
张旺德 550004 贵阳, 贵州医科大学分子生物学重点实验室  
禹文峰 550004 贵阳, 贵州医科大学分子生物学重点实验室  
官志忠 550004 贵阳, 贵州医科大学分子生物学重点实验室  
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中文摘要:
      目的 研究贵州省世居少数民族(布依族、苗族和水族等)人群人类白细胞抗原(HLA)-DP基因rs3077和rs9277535位点多态性与HBV感染的相关性。方法 采用病例-对照研究,募集布依族、苗族和水族等共计256例HBV感染者作为病例组,同时选取142例HBV自限性感染者和135例健康人群作为对照组。应用TaqMan-MGB探针实时荧光定量PCR单核苷酸多态性分型技术对HLA-DP基因rs3077和rs9277535位点进行分型。结果 HBV感染组、HBV自限性感染组和健康对照组相比:HLA-DP基因rs9277535位点的等位基因在HBV感染组和健康对照组之间分布差异有统计学意义(P<0.05),rs3077位点的等位基因和基因型在各组间的差异无统计学意义(P>0.05)。显性模型下各组之间基因型频率比较:HLA-DP基因rs9277535位点在HBV感染组和健康对照组之间的差异有统计学意义(P<0.05),进一步校正年龄、性别因素后显示,AA+AG基因型与GG基因型相比为乙型肝炎(乙肝)易感的保护因素(OR=0.645,95%CI:0.421~0.988);rs3077位点在各组间比较均无统计学意义。性别分层后的显性模型下显示,男性携带rs3077位点CC+CT基因型与TT基因型相比为乙肝易感的保护因素(OR=0.493,95%CI:0.266~0.916);rs9277535位点在显性模型下各组比较基因型的分布差异无统计学意义。在布依族、苗族和水族人群中比较各组中等位基因和基因型分布,HLA-DP基因rs3077位点的基因型在布依族HBV感染组与健康对照组中的分布差异有统计学意义(χ2=6.726,P=0.036)。结论 HLA-DP基因rs9277535位点携带A等位基因可能是乙肝易感的保护因素,男性携带rs3077位点C等位基因可能是乙肝易感的一个保护因素,rs3077和rs9277535位点多态性与HBV的自限性感染可能无关。HBV感染者HLA-DP基因rs3077位点的基因型分布存在民族间的差异。
英文摘要:
      Objective To investigate the association between HLA-DP gene polymorphism and the susceptibility to hepatitis B virus (HBV) infection in people in Buyi, Miao and Shui ethnic groups in Guizhou. Methods A total of 256 patients with HBV infection, 142 HBV self-cleared patients and 135 controls were recruited from 3 ethnic groups for this case-control study. Genotypes of rs3077 and rs9277535 were determined by using real-time PCR with a TaqMan-MGB probe. Results Compared with healthy subjects and self-cleared subjects, the allele distribution of rs9277535 was significantly associated with chronic HBV infection (P<0.05), no significant differences in rs3077 allele and genotype distributions were found among 3 groups (P>0.05). Compared with the rs9277535 GG genotype, the AA and AG genotypes were protective factors against HBV infection in dominant model after adjustment for age and sex (OR=0.645, 95%CI: 0.421-0.988), and no significant difference in rs3077 locus was found in the same analysis (P>0.05). For men, the rs3077 locus CC and CT genotypes were also protective factors against HBV infection (OR=0.493, 95%CI: 0.266- 0.916), and there was no significant difference in rs9277535 locus compared the genotype distributions among 3 groups in dominant mode. Comparison of allele and genotype distribution in 3 ethnic groups showed that HLA-DP gene rs3077 genotype distributions had significant difference between the HBV infection group and the healthy control group in Buyi ethnic group (χ2=6.726, P=0.036). Conclusions The presence of rs9277535A allele at HLA-DP gene polymorphisms might decrease the risk for HBV infection, the rs3077 C allele at HLA-DP gene polymorphisms might also confer protective effect against HBV infection in women. No significant correlation between HLA-DP gene rs3077 and rs9277535 locus polymorphism and HBV clearance was found in this study. The HLA-DP gene rs3077 genotype distribution of HBV-infected patients had significant differences among different ethnic groups.
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