文章摘要
郭晓芳,杨明东,姜进勇,李华昌,朱崇革,桂琴,卜力群,周红宁.云南省中缅边境2015年一起登革热暴发的分子特征分析[J].中华流行病学杂志,2016,37(3):398-401
云南省中缅边境2015年一起登革热暴发的分子特征分析
Molecular characteristics of dengue virus outbreak in China-Myanmar border region, Yunnan province, 2015
收稿日期:2015-08-25  出版日期:2016-03-15
DOI:10.3760/cma.j.issn.0254-6450.2016.03.022
中文关键词: 登革热;登革病毒;暴发
英文关键词: Dengue fever;Dengue virus;Outbreak
基金项目:国家自然科学基金(30960327);中缅老越边境地区疟疾/登革热联防联控项目
作者单位E-mail
郭晓芳 665000 普洱, 云南省寄生虫病防治所云南省虫媒病毒研究中心/云南省虫媒传染病防控研究重点实验室  
杨明东 665000 普洱, 云南省寄生虫病防治所云南省虫媒病毒研究中心/云南省虫媒传染病防控研究重点实验室  
姜进勇 665000 普洱, 云南省寄生虫病防治所云南省虫媒病毒研究中心/云南省虫媒传染病防控研究重点实验室  
李华昌 677000 临沧市疾病预防控制中心  
朱崇革 677500 耿马县疾病预防控制中心  
桂琴 677500 耿马县疾病预防控制中心  
卜力群 677500 耿马县疾病预防控制中心  
周红宁 665000 普洱, 云南省寄生虫病防治所云南省虫媒病毒研究中心/云南省虫媒传染病防控研究重点实验室 zhouhn66@163.com 
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中文摘要:
      目的 对2015年云南省中缅边境一起登革热暴发查明病因,对流行的登革病毒(DENV)进行分子特征分析,为疾病防控提供病原学证据。方法 采用半巢式RT-PCR方法检测2015年7月云南省耿马县孟定镇DENV NS1抗原阳性血清中的DENV特异核酸(CprM基因),对阳性标本用DENV E基因特异引物进行扩增,并将PCR产物送测序,经拼接剪切后的序列在NCBI网站进行BLAST比对,在Megalign中计算核苷酸相似性;在GenBank中选出不同国家和地区不同年度的DENV E基因序列作为参考序列,在Mega 5.05 软件中采用邻接(NJ)法构建系统进化树。结果 对25份中国云南省耿马县孟定镇本地病例和14份缅甸输入病例DENV NS1抗原阳性的血清标本进行DENV特异核酸检测,共有21份本地和10份缅甸输入病例标本为DENV-1阳性,其余8份为DENV阴性。测序得到13株(8株来源于本地病例,5株来源于输入病例)1 485 bp的E基因序列,核苷酸相似性为100%,12株(9株来源于本地病例和3株来源于输入病例)406 bp的CprM基因序列,核苷酸相似性为100%。系统进化分析显示,13株E基因序列均属于DENV-1的基因Ⅰ型,位于独立的进化分支。结论 本次登革热暴发由DENV-1的基因Ⅰ型引起,该病毒与相邻缅甸区域流行的DENV进化关系最近,当地需加强对登革热防控的综合措施,以防止登革热疫情的进一步扩散。
英文摘要:
      Objective To understand the molecular characteristics of a dengue virus outbreak in China-Myanmar border region, Yunnan province, 2015 and provide etiological evidence for the disease control and prevention. Methods Semi-nested RT-PCR was conducted to detect the capsid pre-membrane (CprM) gene of RNA of dengue virus by using dengue virus NS1 positive serum samples collected in Mengdin township, Gengma county, Yunnan province in July, 2015. Some positive samples were then detected by using PCR with specific primers to amplify the full E gene. The positive PCR products were directly sequenced. Then sequences generated in this study were BLAST in NCBI website and aligned in Megalign in DNAstar program. Multiple sequence alignments were carried out by using Mega 5.05 software based on the sequences generated in this study and sequences downloaded from GenBank, including the representative strains from different countries and regions. Phylogenetic trees were constructed by using Neighbor-Joining tree methods with Mega 5.05 software. Results Twenty one of 25 local cases and 10 of 14 imported cases from Myanmar were positive for DENV-1. Eight serum samples were negative for dengue virus. A total of 13 strains with E gene (1 485 bp), including 8 local strains and 5 imported strains, were sequenced, which shared 100% nucleotide sequence identities. Twelve strains with CprM gene (406 bp) from 9 local cases and 3 imported cases shared 100% nucleotide sequence identities. Phylogenetic analyses based on E gene showed that the new 13 strains clustered in genotype Ⅰ of dengue virus and formed a distinct lineage. Conclusions This outbreak was caused by genotypeⅠof DENV-1, which had the closest phylogenetic relationships with dengue virus from neighboring Burma area. Comprehensive measures of prevention and control of dengue fever should be strengthened to prevent the spread of dengue virus.
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