文章摘要
郝海昀,杨志清,许喜喜,王雪飞,王斌,史晓红,付振东,汪波,王素萍.HBsAg阳性母亲HBeAg和新生儿调节性T淋巴细胞关系及对HBV宫内传播的影响[J].中华流行病学杂志,2017,38(10):1410-1414
HBsAg阳性母亲HBeAg和新生儿调节性T淋巴细胞关系及对HBV宫内传播的影响
Relationship between HBeAg from HBsAg positive mothers and regulatory T cells in neonates and its influence on HBV intrauterine transmission
收稿日期:2017-02-15  出版日期:2017-10-23
DOI:10.3760/cma.j.issn.0254-6450.2017.10.023
中文关键词: 乙型肝炎病毒宫内传播;乙型肝炎e抗原;CD4+ CD25+ Foxp3+调节性T淋巴细胞
英文关键词: HBV intrauterine transmission;Hepatitis B e antigens;CD4+ CD25+ Foxp3+ regulatory T cells
基金项目:国家自然科学基金(81072341,81573212);山西省普通高校特色重点学科建设(C01201007)
作者单位E-mail
郝海昀 030001 太原, 山西医科大学流行病学教研室  
杨志清 030001 太原, 山西医科大学流行病学教研室  
许喜喜 030001 太原, 山西医科大学流行病学教研室  
王雪飞 030001 太原, 山西医科大学流行病学教研室  
王斌 030001 太原, 山西医科大学流行病学教研室  
史晓红 030001 太原, 山西医科大学流行病学教研室  
付振东 030001 太原, 山西医科大学流行病学教研室  
汪波 030001 太原市第三人民医院妇产科  
王素萍 030001 太原, 山西医科大学流行病学教研室 spwang88@163.com 
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中文摘要:
      目的 探讨HBsAg阳性母亲的HBeAg与新生儿CD4+CD25+Foxp3+调节性T淋巴细胞(Treg)含量的关系以及对增加新生儿HBV宫内传播发生风险的影响。方法 选择太原市第三人民医院妇产科分娩的270对HBsAg阳性母亲及其新生儿,收集一般人口学特征及分娩情况等资料,采用荧光定量聚合酶链式反应(FQ-PCR)和化学发光免疫试验(CLIA)检测母婴外周血HBV DNA及HBV血清学标志物;流式细胞术(FCM)检测新生儿外周血Treg等免疫细胞含量。结果 母亲HBeAg阳性是HBV宫内传播的危险因素(OR=4.08,95% CI:1.89~8.82);母亲HBeAg阳性者新生儿Treg含量高于阴性者(Z=2.29,P=0.022);按母亲HBeAg滴度分为5组,各组间新生儿Treg、HBeAg及母亲HBV DNA随母亲HBeAg滴度增高呈上升趋势,差异均有统计学意义(χ2=18.73,P<0.001;χ2=181.60,P<0.001;χ2=183.09,P<0.001)。偏相关分析中控制母亲HBV DNA以及新生儿HBeAg后,母亲HBeAg与新生儿Treg呈正相关(rs=0.19,P=0.039)。新生儿Treg与浆细胞样树突状细胞(pDC)、CD4+T淋巴细胞含量为负相关(rs=-0.21,P=0.017;rs=-0.23,P=0.009)。结论 HBsAg阳性母亲的HBeAg可能通过上调新生儿Treg含量,影响树突状细胞和效应T淋巴细胞的功能及其对HBV的免疫应答,从而增加新生儿发生HBV宫内传播的风险。
英文摘要:
      Objective To explore the relationship between HBeAg in HBsAg positive mothers and CD4+ CD25+Foxp3+ regulatory T cells (Treg) in newborns, as well as how they would influence the increasing risk on HBV intrauterine transmission. Methods We collected information on general demographic characteristics and delivery on 270 HBsAg positive mothers and their newborns from the Third People's Hospital of Taiyuan. Fluorescence quantitative polymerase chain reaction (FQ-PCR) and chemiluminescence immunoassay (CLIA) were used to detect HBV DNA and HBV serological markers in peripheral blood from both mothers and neonates. The expression of Treg and other immune cells in peripheral blood of neonates were detected with flow cytometry (FCM). Results Maternal HBeAg positive rates were associated with an increased risk of intrauterine transmission (OR=4.08, 95% CI:1.89-8.82). Rates of Treg in newborns born to HBsAg-positive mothers were higher than that of the negative group (Z=2.29, P=0.022). Each pair of the subjects was assigned to five different groups according to the HBeAg titers of mothers. Frequencies of both Treg and HBeAg in newborns and HBV DNA in mothers between the above said 5 groups showed similar trends of changing patterns and the differences between groups were statistically significant (χ2=18.73, P<0.001; χ2=181.60, P<0.001; χ2=183.09, P<0.001). Results from partial correlation analysis showed that after adjusting for neonatal HBeAg and maternal HBV DNA, mother's HBeAg titers were positively related to the percentage of Treg in their newborns (rs=0.19, P=0.039). In addition, the frequencies of Treg were negatively correlated with pDC and CD4+ T cell in their newborns (rs=-0.21, P=0.017; rs=-0.23, P=0.009). Conclusion HBeAg from HBsAg positive mothers might have inhibited the function of neonatal DC cells and T cells to reduce the immune response to HBV by up-regulating the proportion of Treg and finally increased the risk of HBV intrauterine transmission.
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