| 柯碧霞,曾洪辉,何冬梅,谭海玲,李柏生,梁宇恒,柯昌文.广东省2007-2016年人源沙门菌流行现状及病原学特征[J].中华流行病学杂志,2018,39(1):63-66 |
| 广东省2007-2016年人源沙门菌流行现状及病原学特征 |
| Circulation and etiological characterization of Salmonella enterica serotype in human in Guangdong province, 2007-2016 |
| 收稿日期:2017-05-19 出版日期:2018-01-13 |
| DOI：10.3760/cma.j.issn.0254-6450.2018.01.013 |
| 中文关键词: 沙门菌,1,4,[5],12:i:- 耐药性 分子特征 |
| 英文关键词: Salmonella 1,4,[5],12:i:- Drug resistance Molecular characteristics |
| 基金项目:广东省省级科技计划（2013B060400012，2014A020219004）；中美新发和再发传染病合作项目（1U2GGH000018-01） |
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| 中文摘要: |
| 目的 了解广东省人源沙门菌1，4，[5]，12：i：-的流行现状、耐药性与分子特征。方法 对2007－2016年广东省沙门菌腹泻病例监测检出的人源沙门菌1，4，[5]，12：i：-进行药物敏感试验、PCR检测和PFGE分型。结果 2007－2016年广东省共检出人源沙门菌1，4，[5]，12：i：-2 960株，检出率逐年增多，至2015年成为广东省人源沙门菌中最主要的血清型。病例中男女性别比为1.58∶1，发病年龄以婴幼儿为主。沙门菌1，4，[5]，12：i：-除对亚胺培南100%敏感外，对其他17种抗生素均有不同程度的耐药。2011－2016年对头孢他啶、头孢噻肟和环丙沙星的耐药率呈上升趋势。多重耐药现象严重，具有ASSuT多重耐药的有70.62%（435/616），具有ACSuGSTTm多重耐药的有27.11%（167/616）。第Ⅱ相鞭毛蛋白不表达主要（75.53%）是缺失fljA、fljB和hin基因，但保留了iroB、STM2740、STM2757基因，共有8种不同的缺失方式。2 347株菌有934种PFGE谱型别，表现出较大的指纹图谱多态性，最主要的优势PFGE谱型为JPXX01.GD0226型（97株菌，4.13%）。168株沙门菌1，4，[5]，12：i：-的PFGE图谱与鼠伤寒沙门菌PFGE图谱一致。结论 沙门菌1，4，[5]，12：i：-已成为广东省人源沙门菌中最主要的血清型，且多重耐药现象严重，第Ⅱ相鞭毛蛋白不表达主要由缺失fljA、fljB和hin基因引起，其PFGE型别多样，呈遗传多态性。 |
| 英文摘要: |
| Objective To understand the circulation, drug resistance and molecular characteristics of Salmonella 1,4,[5],12:i:-in human in Guangdong province. Methods Salmonella 1,4,[5],12:i:-isolated from diarrhea patients in Guangdong during 2007-2016 were detected for drug resistance, genes and PFGE characteristics. Results A total of 2 960 strains Salmonella 1,4,[5],12:i:-were isolated from human diarrhea cases during this period. The positive rates of the isolation increased year by year. The male to female ratio of the infection cases was 1.58:1, and the infection mainly occurred in infants and young children. Except imipenem, Salmonella 1,4,[5],12:i:-was resistant to other 17 antibiotics to some extent. The drug resistant rates to ceftazidime, cefotaxime and ciprofloxacin increased from 2011 to 2016. Multi-drug resistance was serious, for example, the multi-drug resistant strains with ASSuT accounted for 70.62% (435/616) and the multi-drug resistant strains with ACSuGSTTm accounted for 27.11% (167/616). The lack of fljA, fljB and hin genes, as well as the retaining of iroB, STM2740, STM2757 genes, resulted in the unable expression of FljBenx gene with 8 different defection profiles. There were 934 different PFGE patterns observed in 2 347 strains, which displayed a relatively large fingerprint polymorphism. The major PFGE pattern was JPXX01. GD0226, which was found in 97 strains, accounting for 4.13% (97/2 347). The PFGE patterns in 168 Salmonella 1,4,[5],12:i:-strains were consistent with that of Salmonella typhimurium. Conclusions Salmonella 1,4,[5], 12:i:-strains has become the major serotype of Salmonella that cause diarrhea in human in Guangdong. The multi-drug resistance of Salmonella 1,4,[5], 12:i:-was serious, and since the defection of fljA, fljB and hin genes, the expression of FljBenx protein failed. The PFGE results were diverse, which displayed polymorphism in inheritance. |
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