文章摘要
柯碧霞,曾洪辉,何冬梅,谭海玲,李柏生,梁宇恒,柯昌文.广东省2007-2016年人源沙门菌流行现状及病原学特征[J].中华流行病学杂志,2018,39(1):63-66
广东省2007-2016年人源沙门菌流行现状及病原学特征
Circulation and etiological characterization of Salmonella enterica serotype in human in Guangdong province, 2007-2016
收稿日期:2017-05-19  出版日期:2018-01-13
DOI:10.3760/cma.j.issn.0254-6450.2018.01.013
中文关键词: 沙门菌,1,4,[5],12:i:-;耐药性;分子特征
英文关键词: Salmonella 1,4,[5],12:i:-;Drug resistance;Molecular characteristics
基金项目:广东省省级科技计划(2013B060400012,2014A020219004);中美新发和再发传染病合作项目(1U2GGH000018-01)
作者单位E-mail
柯碧霞 511430 广州, 广东省疾病预防控制中心病原微生物所  
曾洪辉 510440 广州, 广东省生物制品与药物研究所耐药监测室  
何冬梅 511430 广州, 广东省疾病预防控制中心病原微生物所  
谭海玲 511430 广州, 广东省疾病预防控制中心病原微生物所  
李柏生 511430 广州, 广东省疾病预防控制中心病原微生物所  
梁宇恒 511430 广州, 广东省疾病预防控制中心病原微生物所  
柯昌文 511430 广州, 广东省疾病预防控制中心病原微生物所 kecw1965@aliyun.com 
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中文摘要:
      目的 了解广东省人源沙门菌1,4,[5],12:i:-的流行现状、耐药性与分子特征。方法 对2007-2016年广东省沙门菌腹泻病例监测检出的人源沙门菌1,4,[5],12:i:-进行药物敏感试验、PCR检测和PFGE分型。结果 2007-2016年广东省共检出人源沙门菌1,4,[5],12:i:-2 960株,检出率逐年增多,至2015年成为广东省人源沙门菌中最主要的血清型。病例中男女性别比为1.58∶1,发病年龄以婴幼儿为主。沙门菌1,4,[5],12:i:-除对亚胺培南100%敏感外,对其他17种抗生素均有不同程度的耐药。2011-2016年对头孢他啶、头孢噻肟和环丙沙星的耐药率呈上升趋势。多重耐药现象严重,具有ASSuT多重耐药的有70.62%(435/616),具有ACSuGSTTm多重耐药的有27.11%(167/616)。第Ⅱ相鞭毛蛋白不表达主要(75.53%)是缺失fljA、fljBhin基因,但保留了iroB、STM2740、STM2757基因,共有8种不同的缺失方式。2 347株菌有934种PFGE谱型别,表现出较大的指纹图谱多态性,最主要的优势PFGE谱型为JPXX01.GD0226型(97株菌,4.13%)。168株沙门菌1,4,[5],12:i:-的PFGE图谱与鼠伤寒沙门菌PFGE图谱一致。结论 沙门菌1,4,[5],12:i:-已成为广东省人源沙门菌中最主要的血清型,且多重耐药现象严重,第Ⅱ相鞭毛蛋白不表达主要由缺失fljA、fljBhin基因引起,其PFGE型别多样,呈遗传多态性。
英文摘要:
      Objective To understand the circulation, drug resistance and molecular characteristics of Salmonella 1,4,[5],12:i:-in human in Guangdong province. Methods Salmonella 1,4,[5],12:i:-isolated from diarrhea patients in Guangdong during 2007-2016 were detected for drug resistance, genes and PFGE characteristics. Results A total of 2 960 strains Salmonella 1,4,[5],12:i:-were isolated from human diarrhea cases during this period. The positive rates of the isolation increased year by year. The male to female ratio of the infection cases was 1.58:1, and the infection mainly occurred in infants and young children. Except imipenem, Salmonella 1,4,[5],12:i:-was resistant to other 17 antibiotics to some extent. The drug resistant rates to ceftazidime, cefotaxime and ciprofloxacin increased from 2011 to 2016. Multi-drug resistance was serious, for example, the multi-drug resistant strains with ASSuT accounted for 70.62% (435/616) and the multi-drug resistant strains with ACSuGSTTm accounted for 27.11% (167/616). The lack of fljA, fljB and hin genes, as well as the retaining of iroB, STM2740, STM2757 genes, resulted in the unable expression of FljBenx gene with 8 different defection profiles. There were 934 different PFGE patterns observed in 2 347 strains, which displayed a relatively large fingerprint polymorphism. The major PFGE pattern was JPXX01. GD0226, which was found in 97 strains, accounting for 4.13% (97/2 347). The PFGE patterns in 168 Salmonella 1,4,[5],12:i:-strains were consistent with that of Salmonella typhimurium. Conclusions Salmonella 1,4,[5], 12:i:-strains has become the major serotype of Salmonella that cause diarrhea in human in Guangdong. The multi-drug resistance of Salmonella 1,4,[5], 12:i:-was serious, and since the defection of fljA, fljB and hin genes, the expression of FljBenx protein failed. The PFGE results were diverse, which displayed polymorphism in inheritance.
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