文章摘要
张颖,王超超,牛瑞.利用蛋白质组学技术筛选特发性肺间质纤维化患者血清生物标志物[J].中华流行病学杂志,2018,39(8):1117-1120
利用蛋白质组学技术筛选特发性肺间质纤维化患者血清生物标志物
Screening on serum biomarkers in idiopathic pulmonary fibrosis patients by proteomics technology
投稿时间:2018-01-15  
DOI:10.3760/cma.j.issn.0254-6450.2018.08.020
中文关键词: 特发性肺间质纤维化;蛋白质组学;血清生物标志物
英文关键词: Idiopathic pulmonary fibrosis;Proteomics;Serum biomarkers
基金项目:
作者单位E-mail
张颖 250000 济南, 山东大学第二医院呼吸内科  
王超超 250000 济南, 山东大学第二医院呼吸内科  
牛瑞 250000 济南, 山东大学第二医院呼吸内科 yjxs1127@163.com 
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中文摘要:
      目的 利用蛋白质组学技术筛选并鉴定特发性肺间质纤维化患者和健康人血清差异表达蛋白。方法 收集山东大学第二医院于2014年10月至2015年10月收治的30例特发性肺间质纤维化患者及健康人血清各30份,组内等量混合后,去除高丰度蛋白,经同位素标记相对和绝对定量试剂标记后采用二维液相色谱-串联质谱鉴定并进行相对定量。对鉴定出的差异蛋白进行基因功能聚类(GO)分析和通路富集分析,筛选出与特发性肺间质纤维化发生相关的蛋白质。结果 质谱鉴定出置信度>95%的蛋白质共490个,其中差异表达蛋白25个。与对照组相比,25个差异蛋白中有4个表达上调,21个表达下调。GO分析显示,大多数差异蛋白处于细胞外区域(92%);通路富集分析显示,含差异蛋白数量最多的通路为补体和凝血级联通路。结论 特发性肺间质纤维化患者血清中鉴定出了与补体及凝血相关的差异蛋白,其具体功能有待进一步研究。
英文摘要:
      Objective Applying the proteomics technology to identify proteins differentially in serums of idiopathic pulmonary fibrosis patients and normal population. Methods The study included serum samples from idiopathic pulmonary fibrosis group and normal controlled group with 30 cases of each, from the Second Hospital of Shandong University, between October 2014 and October 2015. Proteins differentially expressed in serums were quantified by the isobaric tags for relative and absolute quantization coupled with two-dimensional liquid chromatography/tandem mass spectrometry technology. The proteins were analyzed in terms of molecular function, cell location and biological processes for showing the key protein molecules which were related to the development of idiopathic pulmonary fibrosis. Results A total of 490 kinds of proteins (with confidence coefficient above 95%) were identified by mass spectrometry and 25 kinds of differentially expressed proteins were found. Compared with the control group, we found 4 types of up-regulated proteins and 21 down-regulated ones in the serum of idiopathic pulmonary fibrosis patients. Data from the Gene ontology analysis showed that most differentially expressed proteins were in the extracellular region (92%) while pathway enrichment analysis showed that most proteins were involved in the complement and coagulation cascade pathway. Conclusion Proteins related to the complement system coagulation cascade pathway, and the proteins function need to be further studied.
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