文章摘要
张慧,刘宇鹏,葛安琪,王玄,孙鸿儒,毕皓然,庞达,赵亚双.外周血白细胞AOX1和IRF4基因甲基化与乳腺癌关系的病例对照研究[J].中华流行病学杂志,2018,39(9):1265-1269
外周血白细胞AOX1和IRF4基因甲基化与乳腺癌关系的病例对照研究
Association between AOX1, IRF4 methylation in peripheral blood leukocyte DNA and the risks of breast cancer: a case-control study
投稿时间:2018-03-29  
DOI:10.3760/cma.j.issn.0254-6450.2018.09.023
中文关键词: 乳腺癌;甲基化;醛氧化酶1;干扰素调节因子4
英文关键词: Breast cancer;Methylation;Aldehyde oxidase 1;Interferon regulatory factor 4
基金项目:国家自然科学基金(81172743)
作者单位E-mail
张慧 150081 哈尔滨医科大学公共卫生学院流行病学教研室  
刘宇鹏 150081 哈尔滨医科大学公共卫生学院流行病学教研室  
葛安琪 150081 哈尔滨医科大学公共卫生学院流行病学教研室  
王玄 150081 哈尔滨医科大学公共卫生学院流行病学教研室  
孙鸿儒 150081 哈尔滨医科大学公共卫生学院流行病学教研室  
毕皓然 150081 哈尔滨医科大学公共卫生学院流行病学教研室  
庞达 150081 哈尔滨医科大学附属肿瘤医院乳腺外科  
赵亚双 150081 哈尔滨医科大学公共卫生学院流行病学教研室 zhao_yashuang@263.net 
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中文摘要:
      目的 探讨外周血中醛氧化酶1(AOX1)和干扰素调节因子4(IRF4)基因甲基化及其和相关环境因素的交互作用与乳腺癌发病的关系。方法 采用病例对照研究,选择2010-2014年纳入的401例乳腺癌病例和555例对照作为研究对象,使用高分辨率熔解曲线法检测AOX1和IRF4基因甲基化水平。应用非条件logistic回归分析基因甲基化水平及其和环境因素的相乘交互作用与乳腺癌的关系,采用Excel软件分析其相加交互作用。结果 AOX1非甲基化的个体发生乳腺癌风险是携带AOX1甲基化个体的1.37倍(95% CI:1.02~1.84)。AOX1甲基化与食用菌类(OR=2.06,95% CI:1.12~3.79)和运动(OR=2.18,95% CI:1.16~4.09)存在协同相乘交互作用,均未发现相加交互作用。IRF4非甲基化能够增加乳腺癌发病风险(OR=1.71,95% CI:0.99~7.43);IRF4甲基化与环境因素无相乘和相加交互作用。结论 AOX1和IRF4非甲基化可能是乳腺癌发生的危险因素。
英文摘要:
      Objective To understand the relationship between AOX1, IRF4 gene methylation status in peripheral blood leukocyte DNA, as well as its interaction with environmental factors, and the risk of breast cancer. Methods A case-control study was conducted among 401 breast cancer patients and 555 cancer-free controls selected from 2010 to 2014. Methylation sensitive-high resolution melting curve analysis was used to detect the methylation status of AOX1 and IRF4. The multiplication interaction effect between genes' methylation and environmental factors on the risk of breast cancer was analyzed by using unconditional logistic regression, and Excel software was used to analyze the additive interaction effect. Results Individuals without AOX1 methylation had a 1.37-fold (95% CI:1.02-1.84) higher breast cancer risk compared to individuals with AOX1 methylation. AOX1 methylation interacted with fungi intake (OR=2.06, 95% CI:1.12-3.79) and physical activity (OR=2.18, 95% CI:1.16-4.09) synergistically, on the risk for breast cancer, but no additive interaction effects were observed. Non-methylation of IRF4 could increase the risk for breast cancer, with statistical significance (OR=1.71, 95% CI:0.99-7.43). Neither multiplication nor additive interactions were observed between IRF4 methylation and environmental factors. Conclusion Non-methylation of AOX1 and IRF4 were a risk factors for breast cancer.
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