文章摘要
王闻卿,崔琪奇,王筱,张勇琪,李彩云,苏靖华,赵冰,黄红,朱林英,许学斌,郝莉鹏.上海市浦东新区食源性小肠结肠炎耶尔森菌耐药及分子流行病学特征[J].中华流行病学杂志,2019,40(3):354-359
上海市浦东新区食源性小肠结肠炎耶尔森菌耐药及分子流行病学特征
Antimicrobial resistance and molecular epidemiology of foodborne Yersinia enterocolitica in Pudong New District, Shanghai
投稿时间:2018-09-29  
DOI:10.3760/cma.j.issn.0254-6450.2019.03.019
中文关键词: 小肠结肠炎耶尔森菌;生鲜食品;耐药;分子型别
英文关键词: Yersinia enterocolitica;Raw food;Antimicrobial resistance;Molecular type
基金项目:上海市浦东新区卫生和计划生育委员会卫生计生科研项目(PW2017A-10)
作者单位E-mail
王闻卿 上海市浦东新区疾病预防控制中心微生物检验科 200136
复旦大学浦东预防医学研究院, 上海 200136 
 
崔琪奇 上海市浦东新区疾病预防控制中心微生物检验科 200136
复旦大学浦东预防医学研究院, 上海 200136 
 
王筱 上海市浦东新区疾病预防控制中心微生物检验科 200136
复旦大学浦东预防医学研究院, 上海 200136 
 
张勇琪 上海市浦东新区疾病预防控制中心微生物检验科 200136
复旦大学浦东预防医学研究院, 上海 200136 
 
李彩云 上海市浦东新区疾病预防控制中心微生物检验科 200136
复旦大学浦东预防医学研究院, 上海 200136 
 
苏靖华 上海市浦东新区疾病预防控制中心微生物检验科 200136
复旦大学浦东预防医学研究院, 上海 200136 
 
赵冰 上海市浦东新区疾病预防控制中心微生物检验科 200136
复旦大学浦东预防医学研究院, 上海 200136 
 
黄红 上海市浦东新区疾病预防控制中心微生物检验科 200136
复旦大学浦东预防医学研究院, 上海 200136 
 
朱林英 上海市浦东新区疾病预防控制中心微生物检验科 200136
复旦大学浦东预防医学研究院, 上海 200136 
 
许学斌 上海市疾病预防控制中心病原微生物鉴定所 200336 xbxu@scdc.sh.cn 
郝莉鹏 复旦大学浦东预防医学研究院, 上海 200136 lphao@pdcdc.sh.cn 
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中文摘要:
      目的 了解上海市浦东新区食源性小肠结肠炎耶尔森菌耐药及分子流行病学特征。方法 2012-2016年主动、定点采集上海市浦东新区4类流通生鲜食品,使用冷增菌方法分离小肠结肠炎耶尔森菌,并分析菌株生物型、血清型、毒力基因型、耐药性和PFGE分子型别。结果 共采集食品3 900份(禽类590份、畜类1 074份、水产品1 488份、蔬菜748份),其中111份(2.8%)检出小肠结肠炎耶尔森菌。禽类制品(5.3%,31/590)和畜类制品(4.5%,48/1 074)的检出率高于水产品(1.6%,24/1 488)和蔬菜制品(1.1%,8/748)。分离株以生物1A型(95.5%)和O:8血清型(42.3%)为主,且分离数与年总分离数呈正相关。所有菌株均缺失4种(ail、ystA、yadAvirF)产毒株标记的毒力基因,76株(68.5%)ystB基因阳性(其中35株属于1A/O:8/ystB)。分离株对氨苄西林(74.8%)和阿莫西林/克拉维酸(70.3%)的耐药率最高,对头孢西丁不敏感率超过50.0%;未发现三代头孢菌素或氟喹诺酮类抗菌药物耐药株,38.7%(43/111)的菌株为多重耐药。O:8和O:5血清型菌株分别存在44和18种PFGE分子型别。结论 上海市浦东新区食源性小肠结肠炎耶尔森菌暴露风险以禽类制品和畜类制品为主,优势菌型为1A/O:8/ystB,虽无典型产毒株特征但仍有潜在致病力。菌株耐药率处于较低水平但存在多重耐药株,PFGE分子型别提示菌株呈高度遗传多样性。
英文摘要:
      Objective To investigate the antimicrobial resistance and molecular epidemiology of foodborne Yersinia (Y.) enterocolitica in Pudong New District of Shanghai. Methods Four kinds of raw food samples were collected in retail circulation sites in Pudong from 2012 to 2016. Cold enrichment method was used to isolate Y. enterocolitica and further detection of biotype, serotype, virulent genes, antimicrobial susceptibility of the isolates and pulsed field gel electrophoresis (PFGE) were conducted. Results A total of 3 900 raw food samples were collected during this period, including poultry product (n=590), livestock product (n=1 074), aquatic product (n=1 488), vegetable (n=748), in which 111 (2.8%) were contaminated by Y. enterocolitica. The detection rates of Y. enterocolitica in poultry product samples (5.3%, 31/590) and livestock product samples (4.5%, 48/1 074) were higher than those in aquatic product samples (1.6%, 24/1 488) and vegetable samples (1.1%, 8/748). The predominant biotype was 1A (95.5%) and predominant serotype was O:8 (42.3%). All the strains lacked ail, ystA, yadA and virF genes, which encoded pathogenic Y. enterocolitica. Seventy six (68.5%) strains harbored ystB gene, in which 35 (31.5%) belonged to 1A/O:8/ystB pattern. Most strains were resistant to ampicillin (74.8%) and amoxicillin/clavulanic acid (70.3%), and non-sensitive rate to Cefoxitin was over 50.0%. No third generation cephalosporin or fluoroquinolone resistant strains were detected, but 38.7% (43/111) strains were multidrug resistant (MDR). Serotype O:8 and O:5 strains had 44 and 18 PFGE patterns, respectively. Conclusions The main foodborne exposure sources of Y. enterocolitica in raw food were poultry and livestock products in Pudong New District. 1A/O:8/ystB was the predominant pattern with potential pathogenicity despite lacks of typical pathogenic virulent genes. The antimicrobial resistant rates of Y. enterocolitica were at a low level, but MDR strains still existed. Molecular types of the isolates showed highly genetic diversity.
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