文章摘要
殷玥琪,陈剑双,成浩,张轩,吴楠楠,陈璐斯,王蓓.无锡市2013-2016年HIV-1亚型及进化分析[J].中华流行病学杂志,2020,41(2):244-248
无锡市2013-2016年HIV-1亚型及进化分析
Transition and evolution of HIV-1 subtype among HIV-1 infections in Wuxi city, 2013-2016
投稿时间:2019-05-13  
DOI:10.3760/cma.j.issn.0254-6450.2020.02.019
中文关键词: HIV-1型;亚型;进化
英文关键词: HIV-1;Subtype;Evolution
基金项目:中央高校基本科研业务费专项资金(2242016K40025);江苏省研究生科研与实践创新计划(KYCX17_0184)
作者单位E-mail
殷玥琪 东南大学公共卫生学院流行病与卫生统计学系, 南京 210009  
陈剑双 东南大学公共卫生学院流行病与卫生统计学系, 南京 210009  
成浩 无锡市新吴区疾病预防控制中心 214028  
张轩 无锡市疾病预防控制中心 214000  
吴楠楠 无锡市第二人民医院医院感染管理科 214002  
陈璐斯 东南大学公共卫生学院流行病与卫生统计学系, 南京 210009  
王蓓 东南大学公共卫生学院流行病与卫生统计学系, 南京 210009 wangbeilxb@163.com 
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中文摘要:
      目的 了解无锡市HIV-1亚型流行及进化特征,为预测本地HIV-1疫情变化提供参考依据。方法 样本来源于2013年4月至2016年7月无锡市部分CD4+T淋巴细胞监测队列,进行HIV-1基因的扩增和测序,采用ChromasPro 1.6和MEGA 7.0软件构建HIV-1序列数据库;采用FastTree 2.1.10和BEAST 1.7.2软件和贝叶斯系统进化推断法重构HIV-1历史传播情况,采用SPSS 22.0软件进行统计学分析。结果 有205例HIV-1感染者,其中≥50岁占32.68%(67/205)。共检测出CRF01_AE、CRF07_BC、CRF67_01B、B、CRF08_BC、CRF68_0B、CRF78_ cpx 7种HIV-1基因型及1例独特重组型。流行亚型以CRF01_AE(51.67%,93/180)及CRF07_BC(17.22%,31/180)为主,不同亚型之间传播方式的差异有统计学意义(χ2=16.99,P≤0.05)。CRF67_01B型(12.78%,23/180)所占比例较高。贝叶斯系统进化推断法分析结果显示,无锡市CRF67_01B型进化率为2.29×10-3,最近共同祖先时间约为2 003.10年,与江苏省及安徽省来源的参考株可能存在亲缘关系,CRF67_01B型于2003年开始在无锡市出现传播。结论 2013-2016年无锡市HIV-1亚型复杂多样,CRF67_01B型已经开始在无锡市流行,应持续监测HIV-1亚型变化,从分子角度为疫情预测提供参考依据。
英文摘要:
      Objective To understand the distribution of HIV-1 subtype in Wuxi city, to predict the local HIV-1 epidemics. Methods Samples were collected from the ‘CD4+T lymphocyte monitoring cohort study’ in Wuxi from April 2013 to July 2016. HIV-1 gene was amplified, sequenced and with HIV-1 sequence database constructed, using both the ChromasPro 1.6 and MEGA 7.0 softwares. Bayesian phylogenetic inference was used to rebuild the history of HIV-1 transmission, while BEAST 1.7.2 and FastTree 2.1.10 software were used for data analysis. Statistical analysis using SPSS 22.0 software. Results Among the 205 subjects of HIV-1 infection, 32.68%(67/205) of them were over 50 years old. Seven subtypes (including CRF01_AE, CRF07_BC, CRF67_01B, B, CRF08_BC, CRF68_0B, CRF78_cpx) and one of the unique recombinant forms (URFs) were detected. The main subtypes were CRF01_AE (51.67%, 93/180) and CRF07_BC (17.22%, 31/180). Differences between subtypes and ways of transmission were statistically significant (χ2=16.99,P≤0.05). The proportion of CRF67_01B (12.78%, 23/180) was higher than before. Results from Bayesian phylogenetic inference analysis showed that the evolution rate was 2.29×10-3 and Time to the Most Recent Common Ancestor (tMRCA) was 2 003.10. CRF67_01B was probably related to the reference strains from Jiangsu and Anhui provinces, and had been spreading in Wuxi since 2003. Conclusions HIV-1 subtypes seemed complex and diverse in Wuxi city in 2013-2016, with CRF67_01B being pervasive. Continuous molecular monitor program was still needed to provide reference for the prediction of epidemics, from the molecular perspective.
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