| 谢彤,吕莉琨,谭昭麟,李力,吕杰,李晓燕.中国天津市一例缅甸输入基孔肯雅热病例的病毒基因分型[J].中华流行病学杂志,2020,41(12):2131-2134 |
| 中国天津市一例缅甸输入基孔肯雅热病例的病毒基因分型 |
| Genotyping on one case with Chikungunya infection introduced into Tianjin in China from Myanmar |
| 收稿日期:2020-01-31 出版日期:2020-12-25 |
| DOI：10.3760/cma.j.cn112338-20200131-00062 |
| 中文关键词: 基孔肯雅病毒 基孔肯雅热 输入病例 蚊媒传染病 系统发生树分析 |
| 英文关键词: Chikungunya virus Chikungunya fever Imported case Mosquito-borne infectious diseases Phylogenetic analyses |
| 基金项目:国家科技重大专项（2017ZX10103007） |
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| 中文摘要: |
| 目的 对天津市1例输入基孔肯雅热病例开展病毒基因分型，确定基孔肯雅病毒（CHIKV）与全球主要流行株的关系。方法 提取临床症状疑似为CHIKV感染患者血清中RNA，采用荧光定量RT-PCR法检测CHIKV核酸。两步RT-PCR法扩增编码CHIKV包膜糖蛋白E1的基因，扩增产物经测序后开展测序分析。将测序结果与全球其他地区流行毒株一同构建系统发生树。结果 天津市输入型CHIKV基因分型属于能够感染白纹伊蚊并在其体内繁殖的东/中/南非基因型印度洋亚型（ECSA-IOL）。系统发生树分析表明，此次输入的CHIKV与2016－2017年在巴基斯坦、意大利、孟加拉国等国家流行的CHIKV毒株高度同源，序列的同源性高达99.4%，并与这些国家流行的CHIKV毒株共同组成1个基因分型簇。结论 此次输入性基孔肯雅热病例感染的毒株更容易在人群中传播，提示应加强对基孔肯雅热输入性病例的防控工作，以防止该蚊媒传染病在我国发生本地聚集性传播。 |
| 英文摘要: |
| Objectives A clinical case caused by Chikungunya virus (CHIKV) was introduced into Tianjin, China from Myanmar. The current study is aimed to phylogenetically analyzing this imported strain and to reveal the relationship between this virus and other circulating CHIKV strains. Methods RNA was extracted from serum of the suspected patient presenting with symptoms compatible with CHIKV infections. Real-time reverse transcription PCR (RT-PCR) assay was used for diagnoses of the patient. For phylogenetic analysis, envelope glycoprotein 1 (E1) gene of CHIKV was amplified by two-step RT-PCR and the products were sequenced. Results The phylogenetic analyses revealed that the imported CHIKV belong to Indian Ocean Lineage (IOL) derived from ECSA genotype and sharing the same cluster with the Aede albopitus-adapted strains that triggered the outbreaks in Pakistan (2016), Italy (2017) and Bangladesh (2017). Conclusion The imported CHIKV strain has the potential to cause explosive outbreaks in China and this event happened in Tianjin calls for strengthening the monitoring programs on mosquito-borne diseases in China. |
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