文章摘要
胡豫杰,许学斌,王艳,王伟,崔鑫楠,何琼玉,李孟寒,徐进,李凤琴.侵袭性非伤寒沙门菌血清型和耐药性分析[J].中华流行病学杂志,2021,42(3):549-554
侵袭性非伤寒沙门菌血清型和耐药性分析
Analysis on serotype and antimicrobial resistance of invasive non-typhoidal Salmonella
收稿日期:2020-03-16  出版日期:2021-03-29
DOI:10.3760/cma.j.cn112338-20200316-00361
中文关键词: 侵袭性非伤寒沙门菌;血清型;耐药性
英文关键词: Invasive non-typhoidal Salmonella;Serotype;Antimicrobial resistance
基金项目:国家重点研发计划(2018YFC1603904);国家科技重大专项(2016ZX08011005)
作者单位E-mail
胡豫杰 国家食品安全风险评估中心国家卫生健康委员会食品安全风险评估重点实验室, 北京 100021  
许学斌 上海市疾病预防控制中心病原微生物所 200336  
王艳 北京积水潭医院检验科 100035  
王伟 国家食品安全风险评估中心国家卫生健康委员会食品安全风险评估重点实验室, 北京 100021  
崔鑫楠 北京农学院食品科学与工程学院 102206  
何琼玉 都柏林大学学院食品安全中心, 都柏林 D04 N2E5  
李孟寒 国家食品安全风险评估中心国家卫生健康委员会食品安全风险评估重点实验室, 北京 100021  
徐进 国家食品安全风险评估中心国家卫生健康委员会食品安全风险评估重点实验室, 北京 100021  
李凤琴 国家食品安全风险评估中心国家卫生健康委员会食品安全风险评估重点实验室, 北京 100021 lifengqin@cfsa.net.cn 
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中文摘要:
      目的 分析7株侵袭性非伤寒沙门菌(iNTS)的血清型和耐药性。方法 针对收集的7株iNTS菌株,开展血清学鉴定、药物敏感性实验以及全基因组测序,并对血清型、MLST型和耐药基因进行鉴定、注释和分析。结果 7株iNTS菌株中,包含1株鼠伤寒血清型和2株Ⅰ 4,[5],12:i:-(ST34型)、2株肠炎血清型、1株科瓦利斯血清型和1株未知血清型Ⅰ 4,[5],12:d:-(ST279型),其中6株为单相菌,沙门菌二相鞭毛基因缺失或假基因化可能有助于增强沙门菌侵袭性。未发现替加环素、氨曲南、阿米卡星及头孢类、碳青霉烯类抗生素耐药株,存在1株八重耐药鼠伤寒沙门菌。耐药基因与耐药表型基本相符。结论 本研究中的iNTS菌株存在八重耐药株,整体耐药水平尚低但不可忽视。
英文摘要:
      Objective To investigate the serotypes and antimicrobial resistance of seven invasive non-typhoidal Salmonella (iNTS) isolates. Methods For 7 iNTS strains collected, serotype identification, antimicrobial susceptibility testing and whole genome sequencing were performed. We identified, annotated and analyzed the serotypes, MLST types, and antimicrobial resistance genes. Results Among the 7 tested iNTS isolates, we found one Salmonella Typhimurium strain and two Salmonella Ⅰ 4,[5], 12:i:-strains whose MLST types were ST34, two Salmonella Enteritidis strains, one Salmonella Corvallis strain and one strain of unknown serotype with the antigenic formulae of Ⅰ 4,[5], 12:d:-(ST279 type). Six of seven strains were monophasic and the deletion or pseudogenization of Salmonella Flagellum gene might contribute to the enhancement of Salmonella invasiveness. None was found to be resistant to tigarcycline, aztreonam, amikacin, cephalosporins and carbapenem and one Salmonella Typhimurium strain was found to be co-resistant to eight classes of antimicrobials at the same time. Resistance genes were generally in accord with relative resistant phenotypes. Conclusion The iNTS strains could show high level multi-drug resistance, indicating that close attention should be paid to the resistance of iNTS though the overall resistance might be relatively not high.
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