| 张楠,张爽,王锋,尹遵栋,苏秋东,张国民,缪宁,毕胜利,王富珍,沈立萍.我国2015-2017年急性乙型肝炎病例HBV基因型分布特征分析[J].中华流行病学杂志,2022,43(6):865-870 |
| 我国2015-2017年急性乙型肝炎病例HBV基因型分布特征分析 |
| Epidemiological distribution of hepatitis B virus genotypes in acute hepatitis B cases in China,2015-2017 |
| 收稿日期:2021-12-30 出版日期:2022-06-16 |
| DOI：10.3760/cma.j.cn112338-20211230-01041 |
| 中文关键词: 急性乙型肝炎 乙型肝炎病毒 基因型 分子流行病学 |
| 英文关键词: Acute Hepatitis B Hepatitis B virus Genotype Molecular epidemiology |
| 基金项目:国家科技重大专项（2017ZX10105015-001-002） |
| 作者 | 单位 | E-mail | | 张楠 | 中国疾病预防控制中心病毒病预防控制所, 北京 102206 | | | 张爽 | 中国疾病预防控制中心病毒病预防控制所, 北京 102206 | | | 王锋 | 中国疾病预防控制中心病毒病预防控制所, 北京 102206 | | | 尹遵栋 | 中国疾病预防控制中心免疫规划中心, 北京 100050 | | | 苏秋东 | 中国疾病预防控制中心病毒病预防控制所, 北京 102206 | | | 张国民 | 中国疾病预防控制中心免疫规划中心, 北京 100050 | | | 缪宁 | 中国疾病预防控制中心免疫规划中心, 北京 100050 | | | 毕胜利 | 中国疾病预防控制中心病毒病预防控制所, 北京 102206 | | | 王富珍 | 中国疾病预防控制中心免疫规划中心, 北京 100050 | wangfz@chinacdc.cn | | 沈立萍 | 中国疾病预防控制中心病毒病预防控制所, 北京 102206 | shenlp@ivdc.chinacdc.cn |
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| 中文摘要: |
| 目的 分析我国急性乙型肝炎（乙肝）病例HBV基因型流行病学分布特征。方法 收集2015-2017年中国疾病预防控制信息系统报告的急性乙肝病例620例，通过巢式PCR扩增获得全长HBV基因组，构建系统发育树确定HBV基因型，结合基础资料分析HBV基因型的分布情况。结果 在620例急性乙肝病例中，成功分型519例（83.71%，519/620），包括A型（0.19%，1/519）、B型（27.17%，141/519）、C型（62.04%，322/519）、D型（9.06%，47/519）、I型（0.77%，4/519）和CD重组型（0.77%，4/519）；2个主要的基因亚型为B2（95.03%，134/141）和C2（72.67%，234/322）。基因型在我国7个地区分布不同，C型在东北（94.55%，52/55）、华北（93.85%，61/65）、华东（78.87%，56/71）和华南地区（58.14%，50/86）的比例较高，B型在华中（58.07%，36/62）和西南（52.94%，45/85）地区的比例较高，西北地区的D型（48.42%，46/95）比例较高；共有515例成功获得血清型，包括adr（57.48%，296/515）、adw（30.87%，159/515）、ayr（0.19%，1/515）、ayw（11.46%，59/515）。B型以adw血清型（92.14%，129/140）为主，C型以adr血清型（91.88%，294/320）为主，D型均为ayw血清型；不同性别、年龄组中，基因型分布差异无统计学意义（P>0.05）。结论 2015-2017年我国急性乙肝基因型以B、C和D型为主，东北、华北、华东和华南地区C型为主；华中、西南地区B、C型均有，B型为主，D型多分布于西北地区；急性乙肝基因型与血清型存在相关性，B型以adw血清型为主，C型以adr血清型为主，D型均为ayw血清型；不同性别和年龄组中急性乙肝基因型分布无差异。 |
| 英文摘要: |
| Objective To analyze the genotype distribution of acute hepatitis B virus in China. Methods A total of six hundred and twenty acute Hepatitis B cases reported to China Information System for Diseases Control and Prevention from 2015 to 2017 were selected. First, the full-length HBV genome was obtained by nested PCR amplification. In addition, the HBV genotype was determined by constructing a phylogeny tree. Finally, using primarydata, HBV genotype distribution was analyzed. Results A total of 519 (83.71%, 519/620) sequences were obtained genotype of 620 acute hepatitis B cases, including A (0.19%, 1/519), B (27.17%, 141/519), C (62.04%, 322/519), D (9.06%, 47/519), I (0.77%, 4/519) and C/D (0.77%, 4/519); B2(95.03%, 134/141) and C2 (72.67%, 234/322) were the two major subgenotypes. Genotypes were distributed differently in seven regions of China. The proportion of genotype C appeared higher in Northeast China (94.55%, 52/55), North China (93.85%, 61/65), East China (78.87%, 56/71), and South China (58.14%, 50/86). The proportion of genotype B was higher in Central China (58.07%, 36/62) and Southwest China (52.94%, 45/85), the proportion of genotype D was the highest in Northwest China (48.42%, 46/95). A total of 515 cases were classified as serotypes, including 'adr' (57.48%, 296/515), 'adw' (30.87%, 159/515), 'ayr' (0.19%, 1/515), and 'ayw' (11.46%, 59/515). Genotype B was dominated by 'adw' serotype (92.14%, 129/140), genotype C was dominated by 'adr' serotype (91.88%, 294/320),all genotype D were 'ayw' serotype. The genotype of acute hepatitis B was correlated with serotype, 'adw' was dominant in genotype B, 'adr' was dominant in genotype C and 'ayw' was dominant in genotype D.In different gender and age group, there was no statistical significance ingenotype distribution (P>0.05). Conclusions The genotype of acute hepatitis B in China from 2015 to 2017 was mainly B, C, and D; genotype C was dominant in the Northeast China,North China, East China and South China; B and C were common in Central and Southwest China, and genotype B was dominant. Genotype D was primarily distributed in Northwest China. The genotype of acute hepatitis B was correlated with serotype, 'adw' was dominant in genotype B, 'adr' was dominant in genotype C and 'ayw' was dominant in genotype D. There was no difference in the distribution of acute hepatitis B genotypes among different genders and age groups. |
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