| 姜婷婷,陈祥生.混合不同个体的样本检测生殖道沙眼衣原体感染准确性的Meta分析[J].中华流行病学杂志,2022,43(12):1995-2001 |
| 混合不同个体的样本检测生殖道沙眼衣原体感染准确性的Meta分析 |
| Accuracy of pooling specimens from different individuals in the detection of genital Chlamydia trachomatis infection: a Meta analysis |
| 收稿日期:2022-05-31 出版日期:2022-12-17 |
| DOI：10.3760/cma.j.cn112338-20220531-00484 |
| 中文关键词: 生殖道 沙眼衣原体 灵敏度 特异度 Meta分析 |
| 英文关键词: Genital Chlamydia trachomatis Sensitivity Specificity Meta analysis |
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| 中文摘要: |
| 目的 对不同个体的样本混合后检测生殖道沙眼衣原体感染的准确性研究进行Meta分析。方法 通过PubMed和Embase数据库收集2022年4月之前发表的混合不同个体的样本检测生殖道沙眼衣原体感染准确性的相关文献。采用质量评价工具QUADAS-2量表进行文献质量评价。利用综合受试者工作特征曲线评估研究间的阈值效应并计算曲线下面积。采用双变量混合效应模型对不同个体的样本混合后诊断生殖道沙眼衣原体感染的灵敏度及特异度进行Meta分析，并进一步根据纳入文献中样本类型、检测技术、混合样本数量进行亚组分析。结果 纳入的18篇文献共调查人数14 846例，其中3篇来自美国，3篇来自加拿大，3篇来自丹麦，2篇来自立陶宛，2篇来自印度，2篇来自荷兰，来自澳大利亚、俄罗斯和新加坡各1篇。通过数据合并分析得到不同个体混合检测对于生殖道沙眼衣原体感染的总体灵敏度为0.98（95%CI：0.97~0.99），总体特异度为1.00（95%CI：1.00~1.00）。亚组分析显示，连接酶链反应技术诊断混合样本的合并灵敏度显著高于PCR技术。结论 基于发表研究可见，与单个个体检测结果相比，混合不同个体的样本检测用于生殖道沙眼衣原体感染的检测具有较高的一致性。 |
| 英文摘要: |
| Objective To determine the accuracy of pooled specimens from multiple individuals for detection of infection with Chlamydia trachomatis. Methods By April 2022, PubMed and Embase searched relevant studies published in peer-reviewed journals. The QUADAS-2 scale of a quality assessment tool was used to assess the quality of the studies. A curve of summary receiver operating characteristic was applied as a comprehensive assessment of diagnosed accuracy. A bivariate mixed-effects model was used for overall value merging in sensitivity and specificity. In addition, the subgroup analyses regarding sample type, testing method, and the number of samples per pool were performed. Results A total of 14 846 subjects were included in the analysis. Three studies were from the United States, three from Canada, three from Denmark, two from Lithuania, two from India, two from the Netherlands, and one from Australia, Russia, and Singapore. Compared with the individual specimens, the pooled specimens of multiple individuals had an overall sensitivity of 0.98 (95%CI: 0.97-0.99) and specificity of 1.00 (95%CI: 1.00-1.00) for the detection of Chlamydia trachomatis. Results from the subgroup showed that the overall sensitivity of ligase chain reaction was significantly higher than that of PCR in the diagnosis of pooled samples. Conclusion It is concluded from the published studies that the pooled specimens were substantially consistent with the single specimens in detecting infection with Chlamydia trachomatis. |
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