| 任孝颖,江玉洁,张雅惠,潘鑫涛,王重建,李琳琳,霍文倩,李玉倩,张振中,刘晓田.血浆外泌体差异表达微小RNA与2型糖尿病的关联研究[J].中华流行病学杂志,2026,47(2):356-361 |
| 血浆外泌体差异表达微小RNA与2型糖尿病的关联研究 |
| Association of differentially expressed microRNAs in plasma exosomes and type 2 diabetes mellitus |
| 收稿日期:2025-06-16 出版日期:2026-02-13 |
| DOI：10.3760/cma.j.cn112338-20250616-00401 |
| 中文关键词: 血浆外泌体 糖尿病， 2型 微小RNA表达谱 病例对照研究 |
| 英文关键词: Plasma exosome Diabetes mellitus, type 2 microRNA profile Case-control study |
| 基金项目:河南省重点研发与推广专项（232102311129）；中国博士后科学基金（2020M672297）；国家自然科学基金青年项目（82003543）；国家重点研发计划“精准医学研究”重点专项（2016YFC0900803） |
| 作者 | 单位 | E-mail | | 任孝颖 | 郑州大学公共卫生学院流行病与卫生统计学系, 郑州 450001 | | | 江玉洁 | 郑州大学公共卫生学院流行病与卫生统计学系, 郑州 450001 | | | 张雅惠 | 郑州大学公共卫生学院流行病与卫生统计学系, 郑州 450001 | | | 潘鑫涛 | 郑州大学公共卫生学院流行病与卫生统计学系, 郑州 450001 | | | 王重建 | 郑州大学公共卫生学院流行病与卫生统计学系, 郑州 450001 | | | 李琳琳 | 郑州大学公共卫生学院流行病与卫生统计学系, 郑州 450001 | | | 霍文倩 | 郑州大学公共卫生学院流行病与卫生统计学系, 郑州 450001 | | | 李玉倩 | 郑州大学药学院临床药学系, 郑州 450001 | | | 张振中 | 郑州大学药学院临床药学系, 郑州 450001 | | | 刘晓田 | 郑州大学公共卫生学院流行病与卫生统计学系, 郑州 450001
河南省肿瘤流行病学重点实验室, 郑州 450001
河南省肿瘤生物标志物与分子成像国际联合实验室, 郑州 450001 | xtliu2008@126.com |
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| 中文摘要: |
| 目的 描述2型糖尿病（T2DM）病例血浆外泌体微小RNA（miRNA）表达谱，探讨miR-122-5p的相对表达水平与T2DM的关联。方法 采用病例对照研究设计，病例组来自河南农村队列2020年随访时依据FPG新诊断的T2DM病例，按照同性别、同村、年龄相差≤3岁的原则，进行1∶1匹配对照。第一阶段发现集纳入5名新诊断的T2DM病例和5名对照，采用小RNA测序分析差异表达miRNA；第二阶段验证集共纳入72名新诊断的T2DM病例及72名对照，通过实时荧光定量PCR检测血浆外泌体miR-122-5p相对表达水平。连续变量采用t检验或Wilcoxon秩和检验，分类变量采用χ2检验比较病例组和对照组之间的分布差异，使用logistic回归和广义线性模型分析血浆外泌体miR-122-5p的相对表达水平与T2DM及糖代谢指标之间的关联。结果 发现集测序发现117个差异表达的miRNA，其中miR-122-5p在T2DM病例中表达上调（log2表达量差异倍数=2.12，P<0.001），且其平均表达量位列首位。验证集发现血浆外泌体miR-122-5p在T2DM病例中的表达量（6.26）高于对照（5.82），差异有统计学意义（P=0.018），调整混杂因素后，外泌体miR-122-5p相对表达水平每升高1个单位，T2DM的患病风险增加40.8%（OR=1.408，95%CI：1.002~1.978），且与胰岛素抵抗标志物甘油三酯葡萄糖指数呈正相关（β=0.115，95%CI：0.009~0.221）。结论 T2DM病例血浆外泌体miRNA表达变化明显，miR-122-5p的相对表达水平在T2DM病例中上调，且与T2DM的患病风险呈正相关。 |
| 英文摘要: |
| Objective To characterize the plasma exosomal microRNA (miRNA) profile in type 2 diabetes mellitus (T2DM) patients and investigate the association of exosomal miR-122-5p expression with the disease. Methods This study was a case-control study, and the case group was drawn from the Henan Rural Cohort and comprised T2DM patients newly diagnosed based on FPG. A 1∶1 matched control group was formed according to the principle of the same gender, same village, and age ± 3 years. The first-phase discovery set included five newly diagnosed T2DM patients and five controls, and the second-phase validation set included 72 newly diagnosed T2DM and 72 controls. miRNA profiling of plasma exosomes was performed by next-generation RNA sequencing in the discovery sets to identify differentially expressed miRNAs. Quantitative real-time PCR was used to detect the relative expression level of plasma exosomal miR-122-5p. Continuous variables were compared between the case and control groups using a t-test or a Wilcoxon rank-sum test, and categorical variables were compared using a χ2 test for distribution differences. The association between plasma exosomal miR-122-5p and T2DM, as well as glucose metabolism indicators, was analyzed using logistic regression and generalized linear models. Results Discovery set sequencing revealed 117 differentially expressed miRNAs, among which miR-122-5p was upregulated in the T2DM patients (log2fold change=2.12, P<0.001), and its average expression level ranked first. The validation set found that plasma exosomal miR-122-5p was upregulated in patients with T2DM (6.26) compared to controls (5.82) with a statistically significant difference (P=0.018). After adjusting for confounding factors, the risk of T2DM increased by 40.8% (OR=1.408, 95%CI: 1.002-1.978) for every 1 unit increase in the relative expression level of exosomal miR-122-5p. It was positively correlated with the insulin resistance marker triglyceride-glucose index (β=0.115, 95%CI: 0.009- 0.221). Conclusions This study demonstrated significant changes in plasma exosomal miRNA in patients with T2DM. Among them, the relative expression level of plasma exosomal miR-122-5p was upregulated in patients with T2DM and was positively correlated with the risk of T2DM. |
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