| 曹九茹,李福星,王欲佳,韩梅,籍静茹,魏俊妮.芳香烃受体及DNA氧化损伤在多环芳烃暴露致胚胎停育中的中介效应分析[J].中华流行病学杂志,2026,47(3):542-548 |
| 芳香烃受体及DNA氧化损伤在多环芳烃暴露致胚胎停育中的中介效应分析 |
| Mediation effect analysis on aromatic hydrocarbon receptors and DNA oxidative damage with embryonic arrest caused by polycyclic aromatic hydrocarbon exposure |
| 收稿日期:2025-07-14 出版日期:2026-03-19 |
| DOI：10.3760/cma.j.cn112338-20250714-00486 |
| 中文关键词: 多环芳烃|胚胎停育|DNA氧化损伤|芳香烃受体|中介效应 |
| 英文关键词: Polycyclic aromatic hydrocarbons|Embryonic arrest|DNA oxidative damage|Aryl hydrocarbon receptor|Mediating effect |
| 基金项目:山西省应用基础研究项目（201901D111206，202503021211148）；山西省卫生健康委科研计划（2020086） |
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| 中文摘要: |
| 目的 探讨多环芳烃（PAHs）暴露与胚胎停育的关联，分析芳香烃受体（AHR）mRNA表达及8-羟基脱氧鸟苷（8-OHdG）水平对该关联的中介效应。方法 采用1∶2匹配病例对照研究，于2021年4月至2022年4月纳入符合胚胎停育诊断标准的92例孕妇为病例组，按1∶2比例匹配同医院同期自愿要求人工流产的184例正常妊娠孕妇作对照组。采集流产绒毛组织检测PAH-DNA加合物、AHR mRNA表达及8-OHdG水平。采用条件logistic回归分析三者与胚胎停育的关联，用R 4.3.3软件Mediation包分析AHR mRNA表达、8-OHdG水平的中介效应。结果 病例组PAH-DNA加合物、AHR mRNA表达及8-OHdG水平均高于对照组，差异有统计学意义（均P<0.001）。中介效应分析显示，8-OHdG水平部分介导了PAHs暴露和胚胎停育的关联，中介效应为0.18（95%CI：0.06~7.42），占比为16.07%。AHR mRNA表达在PAH-DNA加合物与8-OHdG水平之间起中介作用，中介效应为0.74（95%CI：0.07~1.94），占比为27.11%。结论 PAHs暴露是胚胎停育的危险因素，可能通过增加AHR表达引起DNA氧化损伤，进而导致胚胎停育。 |
| 英文摘要: |
| Objective To investigate the association between polycyclic aromatic hydrocarbons (PAHs) exposure and embryonic arrest, and the proportion of mediating effects of aryl hydrocarbon receptor (AHR) mRNA expression and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels on this association. Methods Using a 1∶2 matched case-control study, a total of 92 pregnant women diagnosed with embryonic arrest from April 2021 to April 2022 were selected as the case group, and 184 normal preganant women who voluntarily requested for abortion in the same hospital during the same period were usaed as the control group according to the match ratio of 1∶2. Abortion villus tissues were collected to detect the expression levels of PAH-DNA adducts, AHR mRNA expression and 8-OHdG levels. Conditional logistic regression was used to analyze the association of the three levels with embryonic arrest, and the mediating effects of AHR mRNA expression, and 8-OHdG levels were analyzed with R 4.3.3 software Mediation package. Results The levels of PAH-DNA adducts, AHR mRNA expression, and 8-OHdG levels were higher in the embryonic arrest group than in the control group, and the differences were significant (all P<0.001). The results of mediating analysis showed that 8-OHdG levels partially mediated the association between PAHs exposure and embryonic arrest, with a mediating effect of 0.18 (95%CI:0.06-7.42), accounting for 16.07% of the total. And the expression of AHR mRNA mediated the levels of PAH-DNA adducts and 8-OHdG levels, with a mediation effect of 0.74 (95%CI:0.07-1.94), accounting for 27.11% of the total. Conclusion PAHs exposure is a risk factor for embryonic arrest, which might cause DNA oxidative damage through increased AHR expression, further leading to embryonic arrest. |
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