微量营养素补充对儿童机体抗氧化及DNA氧化损伤影响的研究

张明; 马爱国; 张秀珍; 葛声; 石学香

Abstract

张明,马爱国,张秀珍,葛声,石学香.微量营养素补充对儿童机体抗氧化及DNA氧化损伤影响的研究[J].Chinese journal of Epidemiology,2005,26(4):268-272

微量营养素补充对儿童机体抗氧化及DNA氧化损伤影响的研究

Effect of multiple micronutrients supplementation on anti-oxidative activity and oxidized DNA damage of lymphocytes in children

Received:July 29, 2004

DOI：

KeyWord: 营养素抗氧化 DNA损伤

English Key Word: Nutrient Anti-oxidation DNA damage

FundProject:国家自然科学基金资助项目(30070659)

Author Name	Affiliation
ZHANG Ming	Nutrition Department of Weifang People Hospital, Weifang 261041,China
MA Ai-guo	Institute of Human Nutrition,Medical College of Qingdao University,Qingdao 266021,China.
ZHANG Xiu-zhen	Institute of Human Nutrition,Medical College of Qingdao University,Qingdao 266021,China.
GE Sheng	上海市第六人民医院营养科
SHI Xue-xiang	青岛市疾病预防控制中心

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Abstract:

目的通过营养调查了解农村儿童抗氧化营养素的摄入情况和观察补充五种营养素对儿童机体抗氧化能力及淋巴细胞DNA氧化损伤的影响。方法选择某农村9～11岁健康儿童82名,随机分为补充营养素组(补充组)和对照组,每组41名,采用24h膳食回忆法进行膳食调查。补充组儿童每日补充抗氧化营养素片[含维生素A(VA)600μg、维生素E(VE)100mg、维生素C(VC)300mg、β胡萝卜素(βC)1mg和亚硒酸钠(Se)200μg],而对照组则服用相同颜色和包装的安慰剂,试验期为8周。分别于补充前和补充结束时采集血样,分析两组血浆VA、VE、VC、βC、Se、谷胱甘肽过氧化物酶(GSHPx)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平以及淋巴细胞DNA自发性损伤和H2O2(5、10、25μmol/LH2O2)诱导的氧化损伤。结果膳食调查结果表明,农村儿童VA、VC和硒摄入量偏低,分别为推荐营养素摄入量(RNI)的50.6%、65.6%和67.3%。营养素补充8周后,补充组儿童血浆中βC、VA、VE、VC和Se的水平与对照组相比分别提高了13.4%、32.8%、11.5%、46.9%和24.6%(P<0.01)。GSHPx酶的活性达到161.7酶活力单位/ml,明显高于补充前(100.4酶活力单位/ml)和对照组(110.2酶活力单位/ml)(P<0.01),而血浆中MDA的水平由补充前7.2nmol/ml下降到干预后的4.6nmol/ml(P<0.01)。补充组血浆中SOD水.

English Abstract:

Objective To examine the effect of multiple micronutrients supplementation on anti-oxidative activity and decreasing oxidized DNA damage of lymphocytes in Chinese children. Methods 82 healthy children in a rural areas,aged 9-11 years,were selected and randomized allocated into group receiving supplements and control group with each of them 41. 24-hour dietary recall was used to collect data on daily nutrient intakes of the research subjects.The subjects in the supplement group were given vitamin A(VA) 600 μg, β- carotene( β-C ) 1.0 mg,vitamin E(VE) 100 mg,vitamin C(VC) 300 mg and Na₂SeO₃(Se) 200 μg in a tablet on daily base while those in the control group took a same-sized color placebo tablet. The trial lasted 8 weeks. 5 ml blood samples from each subject were taken during 7 to 9 o'clock in the morning.DNA damage of lymphocytes and levels of plasma VA,VE,VC, β-C,Se,malondialdehyde(MDA), activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) were then analyzed twice before and after the 8- week of trial.Results The low intakes of VA,VC and Se only accounted for 50.6 %, 65.6 % and 67.3 % of their recommended nutrient intake(RNI) respectively.After the trial,levels of plasma β-C,VA,VE,VC and Se in the supplemented group increased by 13.4 %, 32.8 %, 11.5 %, 46.9 % and 24.6 % respectively,compared with the control group, indicating that nutritional status regarding antioxidant nutrients had largely been improved.GSH-Px activity had a significant increase in the supplement group than before the supplement and in the control group ( P 0.01 ). GSH-Px before the trial (the 100.4 U/ml) also showed significant increase after the trial ( 161.7 U/ml)( P 0.01 ).However,the values of SOD and MDA significantly decreased after the trial.Analysis of DNA damage indicated that there was no significant difference in the intrinsic damage of DNA( P 0.05 ). Significant decreases of oxidized DNA damage induced by 5 μmol/L, 10 μmol/L and 25 μmol/L H₂O₂ were found more in peripheral lymphocytes of the supplemented group, than in pre-supplement and the control group after the trial( P 0.01). Conclusion Supplementation of multiple micronutrients could effectively increase the levels of β-C,VA,VE,VC and Se in plasma,and GSH-Px activity.In the meantime,MDA and oxidized DNA damage induced by a low level H₂O₂ decreased significantly after the trial. The reason accounted for the decrease of SOD activity after the trial needs to be further studied.

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