| 褚宸一,何静,王剑波,哈森高娃,张泮河,吴晓明,赵秋敏,蒋宝贵,高燕,曹务春.大兴安岭林区蜱和鼠中莱姆病螺旋体感染及其基因分型研究[J].Chinese journal of Epidemiology,2006,27(8):681-684 |
| 大兴安岭林区蜱和鼠中莱姆病螺旋体感染及其基因分型研究 |
| Investigation on Borrelia burgdorferi sensu lato in ticks and rodents collected in Da Xing-An Mountains Forest areas of China |
| Received:October 21, 2005 |
| DOI： |
| KeyWord: 伯氏疏螺旋体 基因型 蜱 鼠 |
| English Key Word: Borrelia burgdorferi sensu lato Genospecies Tick Rodent |
| FundProject:国家科技攻关计划课题资助项目(2003BA712A05-01);全军“十一五”计划重大专项资助项目(06D006-1) |
| Author Name | Affiliation | E-mail | | CHU Chen-yi | Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China | | | HE Jing | Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China | | | WANG Jian-bo | 内蒙古自治区牙克石林业防疫站 | | | HASEN Gao-wa | 内蒙古自治区牙克石林业防疫站 | | | ZHANG Pan-he | Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China | | | WU Xiao-ming | Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China | | | ZHAO Qiu-min | Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China | | | JIANG Bao-gui | Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China | | | GAO Yan | Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China | | | CAO Wu-chun | Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China | caowc@nic.bmi.ac.cn |
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| Abstract: |
| 目的了解内蒙古大兴安岭林区蜱和鼠中伯氏疏螺旋体的感染及基因分型情况。方法应用巢式PCR扩增蜱和鼠中伯氏疏螺旋体5S-23S rRNA间隔区片段,对阳性产物进行限制性片段长度多态性(RFLP)分析和单链构象多态性(SSCP)分析,RFLP分析显示特殊带型的样本测序分析。结果检测全沟硬蜱1336只,293只阳性,阳性率为21．93％;森林革蜱144只,6只阳性,阳性率为4．17％;嗜群血蜱144只,未发现有伯氏疏螺旋体感染。检测鼠9种145只,感染伯氏疏螺旋体的4种5只,感染率为3．45％;其中检测8只棕背鼫(鼠平),2只阳性。RFLP分析及序列分析显示蜱中有B．garinii20047亚型、B．gariniiNT29亚型、B．afzelii基因型以及不同基因型或亚型伯氏疏螺旋体的混合感染;鼠感染的伯氏疏螺旋体包括B．garinii20047亚型和B．gariniiNT29亚型。SSCP分析结果显示带型多于36种。结论大兴安岭林区蜱及鼠中均存在伯氏疏螺旋体的感染,其中全沟硬蜱的感染率较高;B．garinii型为主要基因型,且该地区伯氏疏螺旋体存在遗传多态性。单只蜱中存在同时感染不同基因型伯氏疏螺旋体的情况,人和宿主动物是否存在不同基因型伯氏疏螺旋体混合感染尚待进一步研究。全沟硬蜱和棕背(鼠平)分别是该林区伯氏疏螺旋体的主要媒介和主要储存宿主。 |
| English Abstract: |
| Objective To detect and study the types of Borrelia burgdorferi sensu lato in ticks and rodents from Da Xing-An Mountains Forest areas of China, Methods Nested PCR was performed to amplify 5S-23S rRNA intergenic spacer of B.burgdorferi.Positive products were analysed by restriction fragment length polymorphism ( RFLP) and single strand conformation polymorphism ( SSCP )，specimens showing unique RFLP profile were sequenced and analysed.Results 1336 lzodes persulcatus，144 Dermacento silvarum， 144 Haemaphysalis concinna and 145 rodents of 9 species were collected from 16 sections of Da Xing-An Mountains Forest areas of China. Specific fragments were amplified from 293 1. persulcatus and 6 D.silvarum and 5 rodents of 4 species. B. burgdorferi was not detected in H, concinna.Among the positively tested 1.persulcatus，209 contained B.garinii genospecies and 45 contained B.叮zelii genospecies based on RFLP. Moreover, B.garinii genospecies consisted of B.garinii 20047 and B.garinii NT29. 17 adult 1.persulcatus were simultaneously infected with B.garinii 20047 and B.garinii NT29. Nine adult 1. persulcatus were simultaneously infected with B.garirtii 20047 and B. afxelii.Four adult I.persulcatus were simultaneously infected with B.garinii 20047 and B.garinii NT29 and B.a fxel ii.Two D.silvarum were infected with B.garinii 20047，1 D. silvarum with B.garinii 20047，2 D.silvarum with B.afzelii.3 rodents were infected with B.garinii 20047 while 2 rodents were infected with B.garinii NT29. Mixed infection was not found in D.silvarum and rodents. In addition, nine 1.persulcatus and one D‘silvarum specimens showed unique RFLP pattern. Data from sequential analysis showed that they all belonged to B‘garinii. PCR-SSCP profiles of SS23S rRNA intergenic spacer of B.burgdorferi in the positive specimens exceeded 36 types; B.garinii 20047 showed 16 types while B.garinii NT29 showing 11 types, B.a_fzelii showing 9 types. SSCP profiles of the specimens coinfected with multiple B.bzzrgdorferi was relatively complex.Conclusion The infection of B.burgdorferi was found in the ticks and rodents in Aa Xing-!1n Mountains Forests areas. The infection rate of I. persulcazus was high. B.garinii was predominant genospecies, and the population of B.burgdorferi was heterogeneous in the area. Mixed infections of different B.burgdorferi genospecies in ticks were found. 1.persulcatus and Clethrionoznys rufocanus were possibly served as major vector and major host for B.burgdorferi，respectively, suggesting that further study is needed to confirm the coinfection in humans and animals in this region. |
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