| 程春林,冯毅,何翔,林鹏,梁淑家,易志强,贺健梅,胡园园,邢辉,范雁,吴士良,邵一呜.中国南方四省区流行的HIV-1 CRFO 1 AE病毒株基因特征研究[J].Chinese journal of Epidemiology,2009,30(7):720-725 |
| 中国南方四省区流行的HIV-1 CRFO 1 AE病毒株基因特征研究 |
| Genetic characteristics of HIV-1 CRFOI AE strains in four provinces of southern China |
| Received:February 26, 2009 |
| DOI：10.3760/cma.j.issn.0254-6450.2009.07.019 |
| KeyWord: 病毒株, HIV-1 CRF01 AE 流行簇 进化分析 |
| English Key Word: HIV-l CRF01一AE strains Epidemic cluster Phylogenetic analysis |
| FundProject:国家高技术研究发展计划(2006AA022418);“艾滋病和病毒性肝炎等霞大传染病防治”科技重大专项课题(2008ZXl0001-004). |
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| Abstract: |
| 目的 分析中周南方四省区HIV-1感染者中流行CRF01 AE病毒株的遗传特征。方法 从广东、广西、江西和湖南省(自治区)2006年新报告HIV-I感染者的血浆样本中提取病毒RNA, 用反转录/巢式PCR方法 扩增gag和erw基因片段, 对获得的CRFOI AE病毒株核酸序列进行系统进化分析, 并通过计算基闪距离和Entropy核苷酸多态性差异方法 分析毒株的遗传特征。结果 从210例CRF01 AE病毒株感染者中, 发现四省区流行的CRF01 AE病毒株存在2个主要的流行簇。流行簇I
共有123例样本, 未发现与之直接相关的凶际参考毒株。流行簇Ⅱ共有57例样本, 与越南CRF01fiE病毒株关系密切, 且存在不同时间样本的混杂。gag和elgv基闪遗传距离分析结果 表明, 流行簇I内基因遗传多样性均明显小于流行簇Ⅱ;核苷酸多态性分析结果 显示, 在gag基因片段42个位点核苷酸组成具有显著差异, erw基渊片段40个位点核苷酸组成存在显著差异;流行簇I相对于流行簇Ⅱ多态性减少的位点上有61个, 多态性增加的位点有21个。结论 在中国南方四省区流行的CRF01—AE病毒株中首次观察到2个独立的流行簇。流行簇I病毒株为该地区最主要的CRF01 AE病毒株, 其流行时间相对较短, 在人群中所占比例较多, 可能是病毒在流行过程中形成的具有传播优势的病毒株。流行簇Ⅱ病毒与来自于越南的CRF01 AE病毒株有较高同源性, 且存在与越南病毒株间的多次传播。 |
| English Abstract: |
| Objective To analyze the genetic characteristics of HIV-1 CRF01 AE strains prevailing in.the four provinces of southern China.Methods Plasma samples were collected from the newly diagnosed HIV-1 individuals reported in 2006 in Guangdong.Guangxi, Jiangxi and Hunan province.The gag and e,gene fragments were amplified from RNA template extracted from plasma using RT and nested PCR Methods .CRFOl AE sequences were analyzed by phylogcnetic Methods and characterized by calculating the genetic distance and Entropy analysis.Results Two main epidemic clusters were found to exist in the CRFOl AE strains from 210 HIV.1 CRF01 AE irifected individuals collected in the 4 provinces.southern China.It was found that no international reference strain was closely co.elated with cluster I, which including 123 samples.The strains in cluster II, consisting 57 cases of samples, were closely related with the strains identified in Vietnam.Genetic distance analysis of gag and env genes showed that the diversity of cluster I Was obviously less than that of cluster II.Data on nucleotide polymorphism showed that nucleotides compositions of 42 sites in gag and 40 sites in eD, V were significantly different between the two clusters.When compared with cluster II, the polymorphism decreased at 6l nucleotide sites but increased at 2 l sites in cluster I.Conclusion ThiS was the firstreport describing that two main epidemic clusters were existed in CRF0 l AE strains revailing in the 4 provinces.Southern China.The virus in cluster 1 was the dominant strain in this region。with shorter period of circulation and higher proportion seen in the HIV-infected population, which might belong to CRF01 AE strain with certain features facilitating the spread of the virus.The virus in cluster 11 wag highly homology with the CRF01 AE strains from Vietnam, and seemed to have had several events of epidemics in populations in border regions of China and Vietnam. |
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