| 董莉娟,陈会超,马艳玲,邢文革.HIV-1 DNA的检测及临床应用[J].Chinese journal of Epidemiology,2022,43(10):1685-1690 |
| HIV-1 DNA的检测及临床应用 |
| Detection and clinical application of HIV-1 DNA |
| Received:December 30, 2021 |
| DOI：10.3760/cma.j.cn112338-20211230-01036 |
| KeyWord: 艾滋病病毒 脱氧核糖核酸 检测 临床应用 |
| English Key Word: HIV DNA Detection Clinical application |
| FundProject: |
| Author Name | Affiliation | E-mail | | Dong Lijuan | Institute for AIDS/STD Control and Prevention, Yunnan Center for Disease Control and Prevention, Kunming 650022, China | | | Chen Huichao | Institute for AIDS/STD Control and Prevention, Yunnan Center for Disease Control and Prevention, Kunming 650022, China | | | Ma Yanling | Institute for AIDS/STD Control and Prevention, Yunnan Center for Disease Control and Prevention, Kunming 650022, China | mayanling68@aliyun.com | | Xing Wenge | National HIV/AIDS Reference Laboratory, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | xingwenge@sina.com |
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| Abstract: |
| HIV-1储存库的持续存在是治愈HIV的主要障碍，在临床研究中，需要可靠的生物标志物对其进行标记。HIV-1 DNA在HIV-1储存库中可被持续检测到，在HIV-1感染诊断、预测病毒反弹和监测治疗效果等方面具有重要应用价值。PCR的检测技术是临床上常用的HIV-1 DNA检测方法，随着技术的不断创新与进步，可更准确地通过定性或定量检测感染细胞中总的、整合的和未整合的HIV-1 DNA。感染细胞中不同形式的HIV-1 DNA作为生物标志物在HIV感染监测和艾滋病治疗相关研究中报道日益增多。本文对感染细胞中HIV-1 DNA的检测方法及其作为生物标志物的临床应用进展进行综述。 |
| English Abstract: |
| The persistence of the HIV-1 reservoir is still the main obstacle to the cure of HIV. In clinical research, reliable biomarkers are needed to label it. HIV-1 DNA can be continuously detected in the HIV-1 reservoir. It has significant application value in diagnosing HIV-1 infection, the timing of antiretroviral therapy, the prediction of virus rebound, and monitoring treatment effects. The detection technology based on polymerase chain reaction (PCR) is the most commonly used HIV-1 DNA detection method in clinical practice. The continuous innovation and advancement of technology can accurately detect the total, integrated, and unintegrated HIV-1 DNA in infected cells using qualitative or quantitative methods. Different forms of HIV-1 DNA in infected cells have been increasingly reported as biomarkers in HIV infection monitoring and AIDS treatment-related research. This article reviews the progress of HIV-1 DNA |
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