单克隆抗体反向被动血凝抑制试验与间接血凝抑制试验在诊断乙脑中的比较

郑锡文; 杨功焕; 张桂云

Abstract

郑锡文,杨功焕,张桂云.单克隆抗体反向被动血凝抑制试验与间接血凝抑制试验在诊断乙脑中的比较[J].Chinese journal of Epidemiology,1987,8(6):368-370

单克隆抗体反向被动血凝抑制试验与间接血凝抑制试验在诊断乙脑中的比较

The Comparison of RPHI with McAbs and HI in the Diagnosis of Japanese Encephalitis

DOI：

KeyWord: 乙脑间接血凝抑制试验反向被动血凝抑制试验

English Key Word: Japanese Encephalitis HI RPHI

FundProject:

Author Name	Affiliation
Zheng Xiwen	Institute of Epidemiology & Microbiology, Chinese Academy of Preventive Medicine, Beijing
et al	Institute of Epidemiology & Microbiology, Chinese Academy of Preventive Medicine, Beijing
张桂云	Institute of Epidemiology & Microbiology, Chinese Academy of Preventive Medicine, Beijing

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Abstract:

本文用乙脑单克隆抗体致敏羊血球的反向被动凝集抑制试验(RPHI),对临床诊断为乙脑患者的124份双份血清进行检测,并与常用的间接血凝抑制试验HI作比较,RPHI的阳性率为83.1%(103/124),HI法的阳性率为79.0%(98/124)。经统计学检验,P>0.05,两法的阳性率无明显差别。再进一步将检测血清按滴度的不同水平分组,进行相关分析,结果表明,两种方法检测的结果存在正相关,且恢复期血清检测结果的相关度高于急性期检测结果的相关度。因此,证明了简便快速的RPHI法可作为诊断乙脑的检测手段。

English Abstract:

In this paper, it has been described to test 124 paired (acute and recovered) sera from the patients who were diagnosed clinically as Japanese Encephalitis by RPHI with McAbs and HI.Results showed that the positive rates of RP HI and HI were 83.1% (103/124) and 79.0% (98/124) respectively. P>0.05. There were little obvious difference between RPHI and HI. Further analysis on different levels of sera titre tested by two methods showed the posititve correlation.
Forthermore, RPHI is easier, quicker than HI. Contrary to HI, it doesn't need to do pretreatment of samples. The results can be read within 30 to 60 minuts. So, it is proved that RPHI is a more suitable method on diagnosing Japanese Encephalitis

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