用二种基因探针对肠毒性大肠杆菌腹泻的初步分子流行病学研究

俞守义; 陈添弥; 陈义忠; 王锡琪; 娄丹; 陈清; 杨芳娣; 吴益民; 周善国; 罗观堤; 黄君健

Abstract

俞守义,陈添弥,陈义忠,王锡琪,娄丹,陈清,杨芳娣,吴益民,周善国,罗观堤,黄君健.用二种基因探针对肠毒性大肠杆菌腹泻的初步分子流行病学研究[J].Chinese journal of Epidemiology,1990,11(3):175-178

用二种基因探针对肠毒性大肠杆菌腹泻的初步分子流行病学研究

A Primary Study on Molecular Epidemiology of Diarrhea Caused by Enterotoxigenic Escherichia Coli by DNA Colony Hybridization Using Three Enterotoxigenic Gene-probes

Received:April 02, 1989

DOI：

KeyWord: ETEC腹泻分子流行病学研究基因探针

English Key Word: ETEC-diarrhea Gene-probe Molecular epidemiology

FundProject:

Author Name	Affiliation
Yu Shouyi	Dept. of Epidemiology, the First Military Medical College
陈添弥	军事医学科学院
陈义忠	Dept. of Epidemiology, the First Military Medical College
王锡琪	广州军事医学研究所
娄丹	沈阳军事医学研究所
陈清	Dept. of Epidemiology, the First Military Medical College
杨芳娣	广州市卫生防疫坫
吴益民	沈阳军事医学研究所
周善国	四川凉山地区卫生学校
罗观堤	广州军事医学研究所
黄君健	广州军事医学研究所

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Abstract:

应用肠毒性大肠杆菌三种肠毒素(LT、ST-P、ST-H)基因探针对近年来从广州、武汉、四川、沈阳、湖南等部分省市搜集到的921株致急性腹泻的大肠杆菌进行DNA-DNA菌落原位杂交检测。LT198株占21.5%(198/921)、ST-H 131株占14.2%(131/921)、ST-P 54株占5.9%(54/92l),ST+LT 19株占2.1%(19/921)。表明我国ETEC腹泻病原体中以LT⁺ ETEC为主,其次为ST-H⁺郊区农村ST-P⁺也占相当比例。为国内ETEC腹泻的分子流行病学研究提供了基本数据和较先进的诊检工具。

English Abstract:

921 isolates of acute-diarrchea-indicing E. Coli strains from several provinces and cities, including Guangzhou, Wuhan.Sichuan, Shenyang and Hunan, have been collected during 1984~1989 and were identified in 1989 for detecting gents coding for these enterotoxins-LT, ST-P, ST-H by DNA colony hybridization using three enterotoxigenic gene probes.Of all the isolates homologous to thesfe genes encoding LT, ST-P, ST-H and both LT and ST were 198 (21.5%), 131 (14.2%), 54 (5.9%) and 18(2.1%), respe-ctivily.It was showed that in our country pathogenic agents of acute infectious diarrheal disease with ETEC strains first might be ETEC-LT strains, secondary ETEC-ST-H strains. Whereas, in rural districts ETEC-ST-P posses higher proportion of ETEC strains.These results provided some essential data and advanced methods for researching further molecular epidemiology of the ETEC diarrheal disease.

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