不同剂量甲型肝炎减毒活疫苗免疫原性与免疫持久性观察

汪萱怡; 马景臣; 张勇; 张英林; 张玉伟; 韩常全; 邢占春; 陈吉朝; 赵守军; 徐志一

Abstract

汪萱怡,马景臣,张勇,张英林,张玉伟,韩常全,邢占春,陈吉朝,赵守军,徐志一.不同剂量甲型肝炎减毒活疫苗免疫原性与免疫持久性观察[J].Chinese journal of Epidemiology,2001,22(2):111-113

不同剂量甲型肝炎减毒活疫苗免疫原性与免疫持久性观察

Immunogenicity and long- term persistence of ant- i HAV in groups with different attenuated and inactived hepatitis A vaccine dosage

Received:October 19, 2000

DOI：

KeyWord: 甲型肝炎疫苗,减毒免疫原性抗体

English Key Word: Hepatitis A Vaccine, attenuated Immunogenicity Antibody

FundProject:国家/ 九五0科技攻关项目( 96 -906 -03 -01)

Author Name	Affiliation
WANG Xuanyi	Department of Epidemiology, Medical Centre of Fudan University, Shanghai 200032, China
MA Jingchen	河北省正定县卫生防疫站
ZHANG Yong	河北医科大学
ZAHNG Yingling	河北省正定县卫生防疫站
ZHANG Yuwei	河北省正定县卫生防疫站
HAN Changquan	河北省正定县卫生防疫站
XING Zhanchun	河北省正定县卫生防疫站
CHEN Jizhao	河北省正定县卫生防疫站
ZHAO Shoujun	Department of Epidemiology, Medical Centre of Fudan University, Shanghai 200032, China
XU Zhiyi	Department of Epidemiology, Medical Centre of Fudan University, Shanghai 200032, China

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Abstract:

目的观察不同剂量的甲型肝炎(甲肝)减毒活疫苗免疫原性与免疫持久性。方法对河北省正定县4个村166名6～8岁事先经血清检测抗HAV阴性的易感儿童，按村分成4组，分别给予0.5、1、2及2剂经60℃3h灭活处理的甲肝减毒活疫苗(滴度为10^6.52TCID50)。并于接种后3、12、24、36及48个月采集观察对象血清，采用AbbottIMxmEIA试剂检测血清抗HAV。并对接种1剂、2剂活疫苗组免后4年部分血清检测中和抗体。结果免后3个月抗体阳转率、抗体几何平均滴度(GMT)同疫苗滴度呈剂量效应关系。免疫后各组抗体水平于免疫后3个月达高峰后逐年下降，12个月内下降较明显，12个月后下降缓慢。免疫后4年接种2剂活疫苗组抗体阳性率与GMT均高于接种0.5剂及1剂组。接种1、2剂活疫苗组部分经AbbottIMxmEIA试剂检测抗HAV阴性的血清检测中和抗体，其阳性率为60%及83%。结论甲型肝炎减毒活疫苗的免疫原性与免疫持久性同疫苗的滴度有关，滴度为10^6.83TCID50的减毒活疫苗具有较理想的免疫原性及免疫持久性。减毒活疫苗接种后近期内不必予以加强免疫。

English Abstract:

Objective T his study was carried out to assess the long -term persistence of antibodies against hepatitis A in different vaccine dosage groups. Methods One hundred and sixty -six susceptibles aged form 6 to 8 years old were selected in Zhengding county, and were divided into 4 different dose groups. Each group received 10 ^{6. 17} TCID 50, 10 ^{6. 52} TCID 50, 10 ^{6. 83} TCID 50 of attenuated hepatitis A vaccine or 10 ^{6. 83} TCID 50 of the same vaccine but treated with 60e for 3 hours. All subjects were bled at month 3,12, 24, 36 and 48. All serum specimens were tested for antibodies to HAV using Abbott-IMx mEIA reagent. Part of the tested negative serum specimens were retested by neutralization assay for 10^{6. 52} TCID 50 and 10 ^{6. 83} TCID 50 doseage groups at 4 years after vaccination. Results The seroconversion rate and GMT 3 months after vaccination in each dosage group showed a significant dose -response relationship. Antibody levels reached the peak at month 3 in each group but declined dramatically within one year and the declineslowed down thereafter. The serocoversion rate and GMT in 10 ^{6. 83} TCID 50 dose group 4 years after vaccination was significant higher than those of other dosage groups. Positive rates of neutralizing antibodies in B and C dosage group were 60. 0% and 83. 3% respectively. Conclusion T he immunogenicity and long -term persistence were related to the attenuated hepatitis A vaccine titer. The vaccine, whose titer was 10^{6. 83} T CID 50, had good immunogenicty and long -term persistence. Booster dose was not needed withinshort time after initial vaccination.

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