| 方立群,张泮河,杨宝安,吴晓明,赵秋敏,刘玮,刘洪,邓永强,詹琳,韩伟国,吕富双,吴劲松,杨红,祝庆余,曹务春.间接免疫荧光抗体试验在传染性非典型肺炎诊断中的应用[J].Chinese journal of Epidemiology,2003,24(6):484-486 |
| 间接免疫荧光抗体试验在传染性非典型肺炎诊断中的应用 |
| The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome |
| Received:May 10, 2003 Revised:June 08, 2012 |
| DOI： |
| KeyWord: 严重急性呼吸综合征 免疫荧光抗体试验 抗体 |
| English Key Word: Severe acute respiratory syndrome Immuno-fluorescence assay Antibody |
| FundProject:国家重点发展计划“863”计划资助项目(2003AA208406) |
| Author Name | Affiliation | | FANG Li-qun | 军事医学科学院微生物流行病研究所, 北京 100071 | | ZHANG Pan-he | 军事医学科学院微生物流行病研究所, 北京 100071 | | YANG Bao-an | 军事医学科学院微生物流行病研究所, 北京 100071 | | WU Xiao-ming | 军事医学科学院微生物流行病研究所, 北京 100071 | | ZHAO Qiu-min | 军事医学科学院微生物流行病研究所, 北京 100071 | | LIU Wei | 军事医学科学院微生物流行病研究所, 北京 100071 | | LIU Hong | 军事医学科学院微生物流行病研究所, 北京 100071 | | DENG Yong-qiang | 军事医学科学院微生物流行病研究所, 北京 100071 | | ZHAN Lin | 军事医学科学院微生物流行病研究所, 北京 100071 | | HAN Wei-guo | 军事医学科学院微生物流行病研究所, 北京 100071 | | LU Fu-shuang | 军事医学科学院微生物流行病研究所, 北京 100071 | | WU Jin-song | 军事医学科学院微生物流行病研究所, 北京 100071 | | YANG Hong | 军事医学科学院微生物流行病研究所, 北京 100071 | | ZHU Qing-yu | 军事医学科学院微生物流行病研究所, 北京 100071 | | CAO Wu-chun | 军事医学科学院微生物流行病研究所, 北京 100071 |
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| Abstract: |
| 目的评价应用间接免疫荧光抗体试验(IFA)在传染性非典型肺炎[严重急性呼吸综合征(SARS)]诊断中的可靠性,探讨SARS患者发病后血清抗体在体内的产生规律。方法采用IFA方法检测不同发病时间的临床确诊SARS病例、疑似病例和其他人群的血清SARS病毒抗体,同时对每一个研究对象采用调查表进行一般情况调查。结果在发病10天内,SARS患者血清IgG阳性率为55.1%,IgM阳性率为16.3%;发病10天后SARS患者IgG阳性率达89.8%,IgM阳性率达65.3%;发病25天以后SARS患者IgG、IgM阳性率均为90.9%。对发病时间与抗体阳性率采用趋势χ2检验,结果显示SARS患者血清IgG、IgM抗体阳性率随着发病时间而上升(IgG趋势检验χ2=16.376,P=0.00005;IgM趋势检验χ2=28.736,P=0.00000)。IFA法用于检测SARS患者发病10天后血清抗体,结果显示灵敏度、特异度及与临床诊断的符合率均在90%以上。结论IFA法适于SARS发病10天后作为实验室辅助诊断方法。 |
| English Abstract: |
| Objective To explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS.Methods Clinically confirmed SARS patients, suspected SARS patients, and controls were included in the study. IFA was used to detect the serum antibody against SARS coronavirus. General information about the subjects was collected using a standard questionnaire.Results The positive rates of specific IgG and IgM against SARS virus within 10 days after onset of the disease were 55.1% and 16.3% respectively and then increased up to 89.8% for IgG and 65.3% for IgM. After 25 days of the onset of the disease, 90.9% patients became positive for both IgG and IgM.Results from chi-square for trend test revealed that the positive rates of both IgG and IgM increased with time (χ2 for trend=16.376, P=0.000 05 for IgG; χ2 for trend=28.736, P=0.000 00 for IgM). Sensitivity, specificity and agreement value of IFA regarding the diagnosis of SARS were all higher than 90%. Conclusions IFA can be used to assist diagnosis of SARS after 10 days of the onset of desease. |
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