| 冯福民,郭梅,刘茜,王东,高宝霞,孙永红,安雅臣,纪春梅.甘露糖结合蛋白基因多态性与肺结核发病关系的研究[J].Chinese journal of Epidemiology,2006,27(12):1082-1085 |
| 甘露糖结合蛋白基因多态性与肺结核发病关系的研究 |
| Study on mannose-binding protein gene polymorphisms and susceptibility to pulmonary tuberculosis |
| Received:March 20, 2006 |
| DOI： |
| KeyWord: 结核,肺 甘露糖结合蛋白基因 病例对照研究 基因多态性 |
| English Key Word: Tuberculosis, pulmonary Mannose-binding protein Case-control study Genetic polymorphism |
| FundProject:河北省卫生厅基金资助项目(04256)；河北省教育厅博士基金资助项目(B2004127) |
| Author Name | Affiliation | | Feng Fu-min | North China Coal Medical College, Tangshan 063000, China | | Guo Mei | North China Coal Medical College, Tangshan 063000, China | | Liu Qian | North China Coal Medical College, Tangshan 063000, China | | Wang Dong | North China Coal Medical College, Tangshan 063000, China | | Gao Bao-xia | North China Coal Medical College, Tangshan 063000, China | | Sun Yong-hong | North China Coal Medical College, Tangshan 063000, China | | An Ya-chen | North China Coal Medical College, Tangshan 063000, China | | Ji Chun-mei | North China Coal Medical College, Tangshan 063000, China |
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| Abstract: |
| 目的探讨甘露糖结合蛋白(MBP)基因多态性与中国人肺结核发病的关系。方法采用病例对照研究，收集肺结核病例125例和健康对照198名的环境因素暴露情况及静脉血，MBP基因多态性分析采用特异引物PCR方法，用SPSS 11.5软件进行危险因素的单因素分析和多因素非条件logistic回归分析。结果病例组和对照组MBP-52、54、57位点的等位基因突变频率分别为8.0％、7.2％、0.4％和5.3％、4.3％、0.5％，按性别分层的Mantel-Haensezl X2分析两组间差异无统计学意义。单因素分析表明体重指数、婚姻状况、卡介苗接种史、卡痕、结核病接触史及家族史、体力劳动、文化程度与肺结核的发生有关。在多因素分析中调整上述危险因素后，总MBP(OR=2.182，95％CI:1.058-4.499)和MBP-52突变型等位基因型(OR=2.574，95％CI:1.028-6.446)与肺结核发病有统计学意义。结论总MBP和MBP-52突变型等位基因可能与肺结核发病有关。 |
| English Abstract: |
| Objective To explore the association between the genetic polymorphisms of mannosebinding protein(MBP) alleles and susceptibility to pulmonary tuberculosis.Methods 125 pulmonary tuberculosis cases and 198 healthy controls were collected.A case-control study was conducted.Threestructural gene mutations in exon 1 of MBP gene(codon 52, codon 54 and codon 57)were studied.Polymerise chain reaction with sequence-specific primers (PCR-SSP) was carried out in the polymorphism in MBP alleles.Information on related risk factors of tuberculosis was collected, using a pre-tested questionnaire.Univariate and multivariate logistic analyses were conducted with SPSS software package. Results The frequencies of mutant heterozygote or homozygote of MBP-52, 54, 57 were 8.0%, 7.2% and 0.4% for cases and 5.3%, 4.3%, 0.5% for controls, respectively.The distribution of mutant genotypes of MBP did not show significant difference between tuberculosis patients and control by Mantel-Haertszel x2 on sex.The univariate analysis demonstrated that body mass index, marital status, vaccinal vestige, bacillus of Calmette-Guerin vaccine immunization, contacted with pulmonary tuberculosis patients, familial traits were the risk factors of pulmonary tuberculosis.After adjusting those related environmental factors in the multivariate logistic analyses, the total MBP(MBP-52, MBP-54 and MBP-57) and MBP-52 heterozygote genotypes were significantly overrepresented in cases, with adjusted OR(95% CI)being 2.182(1.058-4.499) and 2.574(1.028-6.446).Conclusion Total MBP and MBP-52 mutant genotypes might be associated with the susceptibility to pulmonary tuberculosis. |
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