| 曾小云,仇小强,纪龙,余红平.DNA修复基因XPD单核苷酸多态性和环境因素的交互作用与肝细胞癌的关联研究[J].Chinese journal of Epidemiology,2009,30(7):702-705 |
| DNA修复基因XPD单核苷酸多态性和环境因素的交互作用与肝细胞癌的关联研究 |
| Study on the relationship between hepatoceilular carcinoma and the interaction between polymorphisms in DNA repair gene and environmental factors |
| Received:September 16, 2008 |
| DOI：10.3760/cma.j.issn.0254-6450.2009.07.014 |
| KeyWord: 肝细胞癌 XPD基因 遗传多态性 病例对照研究 |
| English Key Word: Hepatocellular carcinoma XPD gene Genetic polymorphisms Case—control study |
| FundProject:国家自然科学基金(30660162);广西科学研究与技术开发计划(桂科攻0719006—2—13);广西青年基金(桂科青0728066). |
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| Abstract: |
| 目的 探讨XPD基因751、312位点单核苷酸多态性和环境因素的交互作用与肝细胞癌的关系。方法 采用以医院为基础的病例对照研究方法 , 运用TaqMan MGB荧光定母实时PCR分析方法 对病例组300例肝细胞癌患者和对照组312例非肿瘤患者进行XPD基因75l和312位点的基因型分析。以非条件logistic同归模型分析比较各基因型在两组中分布频率的差异, 以及基因多态性和环境因素的交互作用。结果 XPD基因751位点3种基因型AA、AC、CC在病例组和对照组中的分布差异无统计学意义(P>0.05), 与携带XPD751野生纯合子AA基因型者比较, 携带XPD75lAC或CC基因型者患肝细胞癌风险无显著增加(P>O.05)。XPD基因312位点3种基因型GG、GA、AA在病例组和对照组巾的分布差异有统计学意义(POR=2.67, 95％CI：0.431～16.537), 但差异尤统计学意义;携带至少一个XPD312, 4等位基因的个体罹患肝细胞癌的风险是GG基阒型的2.62倍(95％P：1.626—4.222), 且差异有统计学意义。交互作用分析结果 表明XPD基因751位点多态性与吸烟、XPD基闪312位点多态性与吸烟、XPD基因312位点多态性与HBsAg阳性之间在肝细胞癌发生中存在交瓦作用, 交互作用的OR值分别为4.291、5.341、7.348。结论 DNA修复基因XPD312A等位基因可能是广西南部地区人群肝细胞癌的危险等位基因, XPD基因多态性与吸烟、HBsAg阳性之间在肝细胞癌发生中存在交互作用.能增加罹患肝细胞癌的风险。 |
| English Abstract: |
| Objective To study the relationship between hepatocellular carcinoma and the interaction of polymorphisms in DNA repair gene XPD with environmental factors.Methods A hospital.based case.control study on hepatocellular carcinoma was conducted.All the hepatocellular carcinoma cases(n=300)were newly diagnosed and controls(n=312)were diagnosed with non-tumor cases.XPD genotype(bys751 Gin and Asp3 1 2 Ash)from blood derived DNA was determined using TaqMan MGB Real.time PCR.Unconditional logistic regression Was used to estimate the odds ratios (ORs)and 95%confidence intervals(c, s).Results For condon 75l genotypes, there was no significant difference between frequencies of the AC or CC among patients and controls(P>O.05)(referent AA).The frequency of XPD3 12A allelic gene Was highel in cases than that in controls and Was associated with all increased risk(adjusted D尺=2.62, 95%C, ：1.626--4.222)for hepatocellular carcinoma when compared with GG genotype.Interactions were fotmd between infection of HBs Ag and3 12(OR=7.348), as well as between smoking and non.wild type gene of D751(OR=4.291)and XPD312 (51341).Conclusion DNA repair D3 12A allelic gene migIlt increase the risk of Hepatocellular careinoma.Interactions between HBsAg infection, smoking and were observed in Hepatocellular carcinoma. |
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