| 孙肖红,张晓龙,刘宇夫,王静,魏莲,高旭,赵文,张勋,杨学兵,张乐.云南省一株版纳病毒的分离和鉴定[J].Chinese journal of Epidemiology,2010,31(6):685-687 |
| 云南省一株版纳病毒的分离和鉴定 |
| Isolation and identification of a Banna virus strain from mosquitoes in Yunnan province |
| Received:November 16, 2009 |
| DOI：10.3760/cma.j.issn.0254-6450.2010.06.019 |
| KeyWord: 版纳病毒 序列分析 |
| English Key Word: Banna virus Sequence analysis |
| FundProject:质检公益科研专项(2007GYJ025) |
| Author Name | Affiliation | E-mail | | SUN Xiao-hong | Chinese Academy of Inspection and Quarantine, Beijing 100123, China | | | ZHANG Xiao-long | Chinese Academy of Inspection and Quarantine, Beijing 100123, China | | | LIU Yu-fu | Mengla Entry-Exit Inspection and Quarantine Bureau | | | WANG Jing | Chinese Academy of Inspection and Quarantine, Beijing 100123, China | | | WEI Lian | Chinese Academy of Inspection and Quarantine, Beijing 100123, China | | | GAO Xu | Chinese Academy of Inspection and Quarantine, Beijing 100123, China | | | ZHAO Wen | Mengla Entry-Exit Inspection and Quarantine Bureau | | | ZHANG Xun | Yunnan Entry-Exit Inspection and Quarantine Bureau | | | YANG Xue-bing | Yunnan Entry-Exit Inspection and Quarantine Bureau | | | ZHANG Le | Chinese Academy of Inspection and Quarantine, Beijing 100123, China | zhang0830@tom.com |
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| Abstract: |
| 目的 对2008年在云南省勐腊县采集的蚊虫进行版纳病毒(BAV)分离和鉴定.方法 2008年7月在勐腊县南嘎村人房和畜圈采集蚊虫标本,用细胞培养法进行虫媒病毒分离并鉴定,测定分离到的BAV第5、8及11节段序列,利用MEGA4软件进行系统进化分析.结果 共采集到蚊虫7种1731只,分离到1株导致C6/36细胞产生规律病变的分离物,经血清学和分子生物学鉴定为BAV,系统进化分析显示第8节段和中国分离株关系最近,而第11节段和越南分离株关系最近.结论 系统进化分析提示该BAV毒株可能与越南分离株之间发生了基因重配. |
| English Abstract: |
| Objective To isolate and identify Banna virus(BAV)from mosquitoes collected in Mengla county of Yunnan province.Methods Mosquito samples were collected in houses and stock yards in Mengla county,2008.Mosquitoes were homogenized and incubated onto both C6/36 and BHK21 cells.The new isolate was identified by using ELISA and RT-PCR.The sequences of segment 5.8 and 11 of BAV were amplified by RT-PCR and determined.Phylogenetic analysis on the new BAV were performed using MEGA4 program.Results 1731 mosquitoes representing 7 species were collected with one strain of BAV isolated and identified.Phylogenetic analysis based on sequences of segment 8 showed the new isolate was closed to BAV strain isolated in Yunnan,but segment 11 sequence was closed to Vietnam strain.Conclusion Results of phylogenetic analysis implied that the BAV re-assortment might have been occurred both in Chinese and Vietnam strains. |
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