| 娄静,刁保卫,李杰,闫梅英,章丽娟,阚飙.沙门菌脉冲场凝胶电泳分型与血清型的对应关系[J].Chinese journal of Epidemiology,2013,34(6):618-621 |
| 沙门菌脉冲场凝胶电泳分型与血清型的对应关系 |
| Correlation between pulsed-field gel electrophoresis profiles and Salmonella serotypes |
| Received:March 20, 2013 Revised:June 05, 2012 |
| DOI： |
| KeyWord: 沙门菌 脉冲场凝胶电泳 血清型 |
| English Key Word: Salmonella Pulsed-field gel electrophoresis Serotype |
| FundProject:国家科技重大专项;中美新发和再发传染病合作项目 |
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| Abstract: |
| 目的分析沙门菌脉冲场凝胶电泳分型(PFGE)与血清分型之间的对应关系.方法选择中国细菌性传染病分子分型实验室监测网络PulseNet China沙门菌监测数据中鼠伤寒(Typhimurium)、肠炎(Enteritidis)、德尔卑(Derby)、阿贡纳(Agona)及山夫登堡(Senftenberg)前五位常见血清型菌株共1230株,进行PFGE聚类分析,以血清分型作为参照进行比对.确定这5种血清型PFGE优势带型的共有条带.结果1230株菌株中,1149株经PFGE预测的血清型与实际血清型一致,PFGE预测血清型的准确率为93.4%.5种血清型经PFGE预测的血清型阳性预测值多在90.0%以上、阴性预测值均在95.0%以上.结论PFGE聚类对5种常见血清型沙门菌血清分型具有较好的提示及验证作用. |
| English Abstract: |
| Objective To analyze the relationship between pulsed-field gel electrophoresis (PFGE) subtyping and serotyping of Salmonella (S.).Methods PFGE was performed and profiles were analyzed on 1230 Salmonella isolates which comprising the top five serotypes including Typhimurium,Enteritidis,Derby,Agona and Senftenberg identified in China.The potential predictive relationship between PFGE banding patterns and particular serotypes was compared and the discriminatory consensus band class markers of individual serotypes were identified.Results Among all the 1230 Salmonella strains,1149 strains were found assistant with serotyping through PFGE cluster analysis,providing the matching accuracy reaching 93.4%.For the five serotypes,the positive prediction rate appeared more than 90.0% and the negative prediction rate was over 95.0% on serotype cluster prediction.Conclusion?Results presented in this study were representatives of the top 5 Salmonella serovars,showing that PFGE cluster analysis could provide clues to identity and confirmation of serotypes. |
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