石晓路,扈庆华,林一曼,邱亚群,李迎慧,江敏,陈琼城.致腹泻奇异变形杆菌病原学及其分子特征研究[J].Chinese journal of Epidemiology,2014,35(6):724-728 |
致腹泻奇异变形杆菌病原学及其分子特征研究 |
Etiological and molecular characteristics of diarrhea caused Proteus mirabilis |
Received:January 05, 2014 |
DOI: |
KeyWord: 奇异变形杆菌 毒力基因 质粒 耐药 |
English Key Word: Proteus mirabilis Virulence gene Plasmid Drug-resistant |
FundProject:国家自然科学基金(81302434);广东省医学科研基金(B2012323);深圳市科技计划项目(201302239);深圳市科创委项目(JCYJ20130329103949651) |
Author Name | Affiliation | E-mail | Shi Xiaolu | Key Laboratory of Infectious Disease Prevention and Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China | shixiaolu831@163.com | Hu Qinghua | Key Laboratory of Infectious Disease Prevention and Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China | | Lin Yiman | Key Laboratory of Infectious Disease Prevention and Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China | | Qiu Yaqun | Key Laboratory of Infectious Disease Prevention and Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China | | Li Yinghui | Key Laboratory of Infectious Disease Prevention and Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China | | Jiang Min | Key Laboratory of Infectious Disease Prevention and Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China | | Chen Qiongcheng | Key Laboratory of Infectious Disease Prevention and Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China | |
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Abstract: |
目的 研究致腹泻奇异变形杆菌病原学特征及毒力基因和质粒携带情况,探讨其与耐药性/致病性的关系。方法 对6 株不同来源(食物中毒、外环境及健康人群)的奇异变形杆菌进行生化、药敏和脉冲场凝胶电泳(PFGE)分析;PCR检测常见的毒力基因;提取质粒后电泳并切胶回收质粒进行测序分析。结果 不同来源的奇异变形杆菌生化特征基本相同,且均具有常见的毒力基因ureC、rsmA、hpmA 和zapA。但各菌株PFGE分型和药敏结果不同,其携带质粒情况也各异。对其中2 株菌存在的小质粒测序后发现,该质粒长2 683 bp,主要编码qnrD 基因,与耐喹诺酮类药物相关。结论 采用传统的生化分析及常见的毒力基因鉴定方法,无法区分不同来源的奇异变形杆菌,可利用PFGE和质粒分析。耐药菌株中普遍存在的质粒与菌株的耐药性相关,存在多个质粒的菌株其耐药性更强。 |
English Abstract: |
Objective To analyze the etiological characteristics,virulence genes and plasmids that carrying diarrhea-causing Proteus mirabilis and to assess their relationship with drug resistance and pathogenicity. Methods Proteus mirabilis coming from six different sources(food poisoning,external environment and healthy people) were analyzed biochemically,on related susceptibility and pulsed-field gel electrophoresis(PFGE). Virulence genes were detected by PCR. Plasmids were extracted and sequenced after gel electrophoresis purification. Results The biochemical characteristics of Proteus mirabilis from different sources seemed basically the same,and each of them showed having common virulence genes,as ureC,rsmA,hpmA and zapA. However,the PFGE patterns and susceptibility of these strains were different,so as the plasmids that they carried. Plasmid that presented in the sequenced strain showed that the 2 683 bp length plasmid encodes qnrD gene was associated with the quinolone resistance. Conclusion Etiological characteristics and molecular characteristics of Proteus mirabilis gathered from different sources,were analyzed. Results indicated that traditional biochemical analysis and common virulence gene identification might be able to distinguish the strains with different sources. However,PFGE and plasmids analysis could distinguish the sources of strains and to identify those plasmids that commonly carried by the drug-resistant strains. These findings also provided theoretical basis for further study on the nature of resistance and pathogenicity in Proteus mirabilis. |
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