| 黄淑敏,于恩庶.蟑螂苍蝇携带小肠结肠炎耶氏菌期限研究[J].中华流行病学杂志,1986,7(6):327-328 |
| 蟑螂苍蝇携带小肠结肠炎耶氏菌期限研究 |
| Study on the Time Limitation of Carrying Yersinia enterocolitica of Periplaneta Americana and Flies |
| 收稿日期: 出版日期:2021-05-29 |
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| 中文摘要: |
通过人工感染实验证明,小肠结肠炎耶氏菌在蟑螂体表带菌为10天左右,体内带菌至少30天,感染后20天还能从粪便检出耶氏菌。苍蝇体表带菌6~7天,体内带菌至少41天。这两种昆虫体内带菌时间均比体表长。说明耶氏菌能在这两种昆虫体内停留甚至繁殖。
检查蟑螂和苍蝇携带耶氏菌,用直接分离培养不易成功,而在改良磷酸缓冲液培养基置4℃增菌2~3周后分离阳性率显著增加。 |
| 英文摘要: |
| The experiments of artificial infection of the cockroaches and flies with Y.enterocolitis showed that, the cockroaches (Periplaneta americana) could carry Y. enterocolitica on their body-surface for about 10 days and in the body-cavity for at least 30 days. Y. enterocolitica could be isolated from fecal specimens of Periplaneta americana up to 20 days after the insects were infected. Y.enterocolitica could be carried by flies for 6 to 7 days on their body-surface and for at least 41 days in their body-cavity. The results proved that Y.enterocolitica could stay, and even reproduce, in the body-cavity of the two kinds of insect. It was found that difficult to isolate Y. enterocolitica from these two kinds of insect by usiug direct cultivation method. The enrichmont of the bactieria in a modified phosphate buffer medium at 4℃, for 2 to 3 weeks could make the isolation successful. |
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