文章摘要
徐迪诚,张澜,赵卓,张根生,刘秉阳,李忠元,张旭帆,文希喆,屠伟英,王泰健,陈尚元.厌氧菌数值鉴定系列培养基的制备和应用[J].中华流行病学杂志,1987,8(1):54-57
厌氧菌数值鉴定系列培养基的制备和应用
A System of Numerical Identification of Anaerobic Bacteria Studies on Preparation & Use of the Culture Media
收稿日期:  出版日期:2021-05-29
DOI:
中文关键词: 
英文关键词: 
基金项目:
作者单位
徐迪诚 哈尔滨市卫生防疫站 
张澜 哈尔滨市卫生防疫站 
赵卓 哈尔滨市卫生防疫站 
张根生 哈尔滨市卫生防疫站 
刘秉阳 中国预防医学科学院流行病学微生物学研究所 
李忠元 中国预防医学科学院流行病学微生物学研究所 
张旭帆 中国预防医学科学院流行病学微生物学研究所 
文希喆 中国兽药监察所 
屠伟英 中国兽药监察所 
王泰健 中国兽药监察所 
陈尚元 中国人民解放军222医院 
摘要点击次数: 458
全文下载次数: 74
中文摘要:
      本文报道使用国产材料及试剂研制出厌氧菌数值鉴定系列培养基,用国产气相色谱仪建立厌氧菌代谢终产物的检查方法,并以厌氧菌的数值鉴定原理、操作方法、药敏试验、厌氧菌的新种及厌氧菌常见属种编码等为内容编辑出《厌氧菌数值鉴定指南》,在我国首次建立厌氧菌的简易鉴定系统。
用常规厌氧菌鉴定法为对照,使用这个鉴定系统检查64株标准厌氧菌和277株从临床感染症分离到的厌氧菌。鉴定结果,属水平的鉴定符合率为96%,种水平的鉴定符合率为76%,与法国的API-20A的鉴定效果一致。经北京、长春、南昌等地的六个科研、生产及医疗单位考核验证,共同认为:这个厌氧菌鉴定系统操作简便,出报告结果的时间快,费用低廉,适合医学、兽医学、科研教学单位的中小型实验室使用。
英文摘要:
      The paper presents the initial establishment in China an system for numeric l identification of anaerobic b cteria (NISA). The system consists of preparing serial culture media with n tional biochemicals for use in identification tests, utilizing Chinamade gas chromatography system in analysing the end metabolites, nd publishing a Manu l on (Theory & Practice of Numeric l System for Identifying An erobes) comparable to that of API-20A.
For evaluation of the proposed system, 64 strains of known anaerobes (type strains from CDC. VPI included) & 277 strains of anaerobic b cteria clinically isolated in China were identified with our system s compared with controls by utilizing the routine standard techniques for identifying anaerobes. A correlation of 96% was obt ined at the level of Genera and 76% as species. These results of identifying anaerobes compared favorably with those obtained from API-20A.
The results of evaluation prove that the proposed NISA in china possess the advantages of simplicity in application, rapidity in yielding laboratory report, inexpensive in cost, and suitable for clinical & laboratory use in assisting medical & veterinary diagnosis, and for te ching purposes in microbiology.
查看全文   Html全文     查看/发表评论  下载PDF阅读器
关闭