文章摘要
李国庆,郭可謇,李性善,秦耀洲,高惠敏,许文波,张礼壁.用ELISA法评定麻疹的免疫状态[J].中华流行病学杂志,1989,10(2):105-108
用ELISA法评定麻疹的免疫状态
A Study on Measies immune Status by ELISA
收稿日期:  出版日期:2021-06-01
DOI:
中文关键词: 酶联免疫吸附法  麻疹免疫状态  预测值
英文关键词: ELISA  Measles immune status  Predictive value
基金项目:
作者单位
李国庆 河北省卫生防疫站 
郭可謇 中国预防医学科学院病毒学研究所 
李性善 河北省卫生防疫站 
秦耀洲 河北省卫生防疫站 
高惠敏 河北省卫生防疫站 
许文波 中国预防医学科学院病毒学研究所 
张礼壁 中国预防医学科学院病毒学研究所 
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中文摘要:
      本文报告了应用ELISA法和HI法检测麻疹流行前后85对血清的结果。表明:流行前抗体滴度HI<1:2、ELISA<1:100的22例儿童全部发病。另两例,一例HI<1:2、ELISA1:100。一例HI1:2、ELISA<1:100的儿童也发病。5例流行前抗体滴度HI1:2~1:8,ELISA1:100~1:400者,3人发病,2人隐性惑染。流行前抗体滴度HI≥1:16、ELISA≥1:400的56例接触者既未发病,也无抗体升高。两种方法比较表明:当ELISA<1:200为抗体阴性时,ELISA法的灵敏度为100%,特异度为95.2%。ELISA法可以代替HI法进行麻疹的免疫状态监测。
英文摘要:
      Both ELISA and HI tests have been used to test 85 paired sera collected shortly before and after a measles epidemic. Results showed that 22 children whose pre-exposure antibody titers were<1:2 by HI and <1:100 by ELISA contracted measles subsequently and other 2 children, one<1:2 by HI and 1:100 by ELISA, one 1:2 by HI and<1:100 by ELISA contracted too. 3 of 5 children whose preexposure antibody titers ranged from 1:2 to 1:8 by HI and 1:100 to 1:400 by ELISA, contracted the disease and 2 of them developed a subclinical infection. The antibody titers of 56 persons with pre-exposure was 1:16 by HI aud ≥ 1:400 by ELISA neither contracted measles nor displayed an antibody enhancement. The comparison between the two tests showed that when the antibody titer of ELISA<1:200 was regarded as a negative mark, the sensitivity of this test was 100% and specificity was 95.2%.The ELISA can be used in place of HI test for immune status surveillance against measles.
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