| 卢德威,王大勇,严延生,姚虹,于恩庶,尚德秋,崔春槐,李兰玉.牛布氏菌病特异抗体的鉴别[J].中华流行病学杂志,1989,10(3):143-145 |
| 牛布氏菌病特异抗体的鉴别 |
| An Identification of Special Antibodes for Brucellosis |
| 收稿日期: 出版日期:2021-06-01 |
| DOI： |
| 中文关键词: 布氏菌病 免疫斑点试验 小肠结肠炎耶氏菌 |
| 英文关键词: Brucella Y. enterocolitica Dot-ELISA |
| 基金项目: |
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| 摘要点击次数: 1746 |
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| 中文摘要: |
| 应用硝酸纤维素膜为固相的免疫斑点试验,以牛种布氏菌和0:3型小肠结肠炎耶氏菌为抗原,检查88份布病疫区牛血清,并与常规ELISA和试管凝集试验进行比较,结果表明免疫斑点试验与常规ELISA非常一致,符合率达到98%,均比试管凝集试验敏感。免疫斑点试验还能鉴别布氏菌抗体和0:9型小肠结肠炎耶氏菌抗体,是其它方法不能比的,加上方法简便、快速、稳定、容易掌握,具有在基层推广的条件,可以帮助临床诊断和流行病学调查。 |
| 英文摘要: |
| Appling antigens of B. abortus and Y. enterocolitica 0:3, detection 88 sera of cattle in foci of Brucellosis was carried out by Dot-ELISA on nitrocellulose filter paper, and compared with conventional ELISA and SAT. The results showed that coincident rate of Dot-ELISA and ELISA was 98%. Their sensitivity was more than SAT. In contrast with others, the Dot-ELISA sould differentiate Brucella antibody from antibody to Y. enter, 0:9. In addition, the method is a simple, Speed, stable, and very easy. The procedure sould help clinical diagnosis and epidemiological investigation. |
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