| 万康林,张金声,张哲夫.清除莱姆病螺旋体培养物中杂菌的方法研究[J].中华流行病学杂志,1991,12(5):299-301 |
| 清除莱姆病螺旋体培养物中杂菌的方法研究 |
| Study on the Elimination of Bacteria from the Cultures of Lyme Disease Spirochetes (Borrelia burgdorferi) |
| 收稿日期:1990-06-23 出版日期:2021-06-09 |
| DOI： |
| 中文关键词: 莱姆病螺旋体 污染 纯化 |
| 英文关键词: Lyme disease spirochetes Contamination Purification |
| 基金项目: |
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| 摘要点击次数: 1208 |
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| 中文摘要: |
| 应用抗血清法、药物法、稀释法、微孔滤膜自动过滤法和动物接种法对从蜱分离的伴有较多杂菌的H11和H20莱姆病螺旋体培养物和从病人分离的伴有少量杂菌的R9莱姆病螺旋体培养物进行了纯化。抗血清法对污染严重的培养物可除去大部分杂菌,达到初步纯化。初步纯化的莱姆病螺旋体培养物和伴有少量杂菌的莱姆病螺旋体培养物,用滤膜过滤法或药物法或稀释法或动物接种法均可达到纯化目的。但过滤法最简便、效果最好。 |
| 英文摘要: |
| Contaminated cultures of strains H11, H20 from ticks, and strain R9 from patient were purified by antisera for bacteria, chemicals, filtzation dilation and animal inoculation. Most of bacteria, were eiminated after adding antisera into contaminated strains H11, H20 The preliminarily decontaminated cultures of strains H11, H20 or those with a few bacteria such as strain R9 could be completely purified by filtration, chemicals, dilution, or animal inoculation, but filtration is the simplest and the most effective purification method. |
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