李爱萍,谢慰杖,赵世立,陈伟,宋伟涛,王宇露,王少军.海捕毛蚶中分离到甲型肝炎病毒[J].中华流行病学杂志,1992,13(3):141-142 |
海捕毛蚶中分离到甲型肝炎病毒 |
Isolation of HAV from Sea Clam |
收稿日期:1991-08-15 出版日期:2021-06-05 |
DOI: |
中文关键词: HAV 毛蚶 |
英文关键词: HAV Sea Clam |
基金项目: |
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摘要点击次数: 2012 |
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中文摘要: |
应用电镜和超薄切片技术、免疫荧光(IF)试验、酶联免疫试验、核酸分子杂交技术、病毒感染滴度测定及中和试验等方法,直接从海捕毛蚶中检测和分离到三株甲肝病毒(HAV),并在人二倍体(2BS)细胞上稳定传代,感染滴度在4.0~5.0TCID50/ml之间。初步结果证实,海捕毛蚶受污染并携带甲肝病毒,可成为甲肝流行的潜在危险因素。 |
英文摘要: |
Three strains of HAV were isolated directly from clams (Area Subcrensta Lisckke) collected from sea with cell culture and identified by electron microscopy, immunofluorescent test, ELISA, HAV-cDNA hybridization and neutralization test.The strains were stably passed in 2BS cells with the titer of 4.0~5.0 TCID50/ml. Results indicated that the clams were contaminated by HAV and may cause an outbreak of hepatitis A. |
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