刘明团,王树声,朱田风,韦一知.PCR系统制备地高辛标记的探针检测脊髓灰质炎病毒核酸[J].中华流行病学杂志,1997,18(3):164-166 |
PCR系统制备地高辛标记的探针检测脊髓灰质炎病毒核酸 |
Detection of Poliovirus by Digoxigenin-labeled cDNA Probe Prepared by PCR Technique |
收稿日期:1997-01-06 出版日期:2021-06-09 |
DOI: |
中文关键词: PCR 地高辛标记 核酸斑点杂交 |
英文关键词: PCR Digoxigenin-labeling Dot hybridization |
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中文摘要: |
笔者应用聚合酶链反应(PCR)合成系统,以逆转录的SabinⅠ、Ⅱ、Ⅲ型病毒cDNA为模板,在反应液中加入标记的Dig-dUTP,经扩增制备了地高辛配基标记的脊髓灰质炎病毒cDNA探针、该法比PCR扩增产物后,电泳,片段回收到标记、提纯,常需3天。结果比较PCR技术直接制备地高辛标记cDNA探针方便,快速,标记率高,用于型别鉴定比中和试验快,敏感,特异性强等优点。 |
英文摘要: |
A study including reverse transcribed cDNAs of Sabin Ⅰ, Ⅱ, Ⅲ virus used as templates and digoxigenin-labeled dUTP added to the reaction solution was carried out. After amplification, digoxigenin-labeled poliovirus cDNA probe was prepared by this PCR technique. Results showed that the direct preparation of digoxigenin-labeled poliovirus cDNA probe by PCR is a convenient and rapid method with a high labeling rate. Compared with neutralization test, the probe has the advantages of more rapid,more sensitive and more specific for poliovirus typing. |
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