文章摘要
刘明团,王树声,朱田风,韦一知.PCR系统制备地高辛标记的探针检测脊髓灰质炎病毒核酸[J].中华流行病学杂志,1997,18(3):164-166
PCR系统制备地高辛标记的探针检测脊髓灰质炎病毒核酸
Detection of Poliovirus by Digoxigenin-labeled cDNA Probe Prepared by PCR Technique
收稿日期:1997-01-06  出版日期:2021-06-09
DOI:
中文关键词: PCR  地高辛标记  核酸斑点杂交
英文关键词: PCR  Digoxigenin-labeling  Dot hybridization
基金项目:
作者单位
刘明团 广西壮族自治区卫生防疫站 南宁 530021 
王树声 广西壮族自治区卫生防疫站 南宁 530021 
朱田风 广西壮族自治区卫生防疫站 南宁 530021 
韦一知 广西壮族自治区卫生防疫站 南宁 530021 
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中文摘要:
      笔者应用聚合酶链反应(PCR)合成系统,以逆转录的SabinⅠ、Ⅱ、Ⅲ型病毒cDNA为模板,在反应液中加入标记的Dig-dUTP,经扩增制备了地高辛配基标记的脊髓灰质炎病毒cDNA探针、该法比PCR扩增产物后,电泳,片段回收到标记、提纯,常需3天。结果比较PCR技术直接制备地高辛标记cDNA探针方便,快速,标记率高,用于型别鉴定比中和试验快,敏感,特异性强等优点。
英文摘要:
      A study including reverse transcribed cDNAs of Sabin Ⅰ, Ⅱ, Ⅲ virus used as templates and digoxigenin-labeled dUTP added to the reaction solution was carried out. After amplification, digoxigenin-labeled poliovirus cDNA probe was prepared by this PCR technique. Results showed that the direct preparation of digoxigenin-labeled poliovirus cDNA probe by PCR is a convenient and rapid method with a high labeling rate. Compared with neutralization test, the probe has the advantages of more rapid,more sensitive and more specific for poliovirus typing.
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