文章摘要
杨红彦,陈淑芬,纪绍忠,孟宗达,康志刚,张忠,齐顺祥.一株新轮状病毒引起河北省石家庄市成人腹泻爆发流行[J].中华流行病学杂志,1998,19(6):336-337
一株新轮状病毒引起河北省石家庄市成人腹泻爆发流行
A Novel Rotavirus Causing Large Scale of Adult Diarrhea in Shi Jiazhuang
收稿日期:1998-08-24  出版日期:2014-09-18
DOI:
中文关键词: 成人腹泻  新轮状病毒  RT-PCR
英文关键词: Adult diarrhea  Novel Rotavirus  RT-PCR
基金项目:
作者单位
杨红彦 中国预防医学科学院流行病学微生物学研究所,北京 102206 
陈淑芬 河北省卫生防疫站 
纪绍忠 中国预防医学科学院流行病学微生物学研究所,北京 102206 
孟宗达 河北省卫生防疫站 
康志刚 中国预防医学科学院病毒学研究所 
张忠 河北省卫生防疫站 
齐顺祥 河北省卫生防疫站 
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中文摘要:
      1997年4月10~28日,在河北省石家庄市某高校发生一起成人急性腹泻的爆发流行,累积1000多名大学生发病。经不连续聚丙烯酰胺凝胶(PAGE)电泳检查腹泻病人粪便标本核酸图谱一致呈4-2-1-1-1-1-1排列的新轮状病毒(新RV)14份,阳性率占47%(共检标本30份),且未见其它带型的轮状病毒。提示该核酸图谱的轮状病毒是此次成人急性腹泻爆发流行的主要病原。用已知ADRV第5及第9基因片段末端引物进行逆转录-聚合酶链反应(RT-PCR),结果阳性对照ADRV的扩增结果都为阳性,而新RV的扩增结果都为阴性。这些结果表明该新RV不属于B组轮状病毒,是与成人腹泻轮状病毒(ADRV)完全不同的新RV。非ADRV的轮状病毒引起成人腹泻爆发流行在河北省尚属首次。
英文摘要:
      An outbreak of adult diarrhea causing more than 1 000 hospitalized cases among graduates in a university in Shi Jiazhuang city (Hebei province),occurred in 10-28,April,1997. The non-bacterial pathogenic agent is confirmed as Rotavirus first by PAGE. Forteen samples have been demonstrated sharing the same nucleic acid electrophoresis pattern of 4-2-1-1-1-1-1. The positive rate was 47% (thirty samples have been checked) with no other RV pattern. Dada showed that RV with this kind of nucleic acid electrophoresis pattern were responsible for the outbreaks acute diarrhea in adults. The novel RV and known ADRVwas RT-PCR with end-primers 5, 9 of typical ADRV, the positive control typical ADRV's results are positive, the novel RV's are negative. Experimental results showed that the novel RV did not belong to group B RV, totally different from all the known ARDV. This case was the first reported one in Hebei province with an outbreak of adult diarrhea not by any known ADRV.
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