文章摘要
白石山,李秀霞,夏桂枝,苑宏,郑文艳,王红.福氏2a志贺氏菌肠毒素基因分型[J].中华流行病学杂志,2000,21(4):280-282
福氏2a志贺氏菌肠毒素基因分型
Gena types of Shigella enterotoxins of S.flexneri 2a
收稿日期:1999-11-26  出版日期:2014-09-16
DOI:
中文关键词: 福氏志贺氏菌;肠毒素;基因;聚合酶链反应
英文关键词: Shigella flexneri;Enterotoxins;Gene;Polymerase chain reaction (PCR)
基金项目:
作者单位
白石山 解放军第二五三医院传染科, 呼和浩特 010051 
李秀霞 内蒙古医学院病理教研室 
夏桂枝 南京军区福州总医院儿科 
苑宏 北京军区卫生部 
郑文艳 解放军第二五三医院传染科, 呼和浩特 010051 
王红 第一军医大学流行病学教研室 
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中文摘要:
      目的 对福氏2a志贺氏菌(Shigella flexneri 2a,F2a)的志贺氏肠毒素1(Shigella enterotoxin 1,SET1)和志贺氏肠毒素2(Shigella enterotoxin 2,SET2)进行基因分型,提高菌痢暴发流行时同源性分析的水平。方法 对一起暴发F2a菌痢在现场流行病学调查的基础上,用聚合酶链反应(PCR)方法,检测43株F2a菌的志贺氏肠毒素1基因(set 1)和志贺氏肠毒素2基因(set 2),进行基因分型和同源性鉴定。 结果 43株F2a菌株分为3种基因型,即1株set 1(-)/set 2(+);3株set 1(+)/set 2(-);39株set 1(+)/set 2(+)。结论 本次菌痢暴发是经污染食物传播,暴发事件中夹杂着散发病例,set 1/set 2基因分型对F2a菌痢暴发流行中的同源性鉴定具有重要作用。
英文摘要:
      Objective To identify homogenous clones of S.flexneri 2a, using gene distribution polymorphism of Shigella enterotoxin 1(set 1) and Shigella enterotoxin 2(set 2) in a Shigellosis outbreak caused by S.flexneri 2a. Methods In addition to field epidemiological investigation,43 strains of S.flexneri 2a were determined by polymerase chain reaction(PCR). Results The 43 strains (1 from food, 1 from a carrier, 41 from patients) were divided into 3 clones(set1+/set2+ for 39 strains, set1-/set2+ for 1,set1+/set2- for 3 strains). Conclusion This outbreak was caused by S.flexneri 2a. The route of transmission was contaminated-food borne. Some sporadic cases were noticed during the outbreak. The method used in this study was satisfactory to the identification of S.flexneri 2a.
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