| 白石山,李秀霞,夏桂枝,苑宏,郑文艳,王红.福氏2a志贺氏菌肠毒素基因分型[J].中华流行病学杂志,2000,21(4):280-282 |
| 福氏2a志贺氏菌肠毒素基因分型 |
| Gena types of Shigella enterotoxins of S.flexneri 2a |
| 收稿日期:1999-11-26 出版日期:2014-09-16 |
| DOI： |
| 中文关键词: 福氏志贺氏菌 肠毒素 基因 聚合酶链反应 |
| 英文关键词: Shigella flexneri Enterotoxins Gene Polymerase chain reaction (PCR) |
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| 中文摘要: |
| 目的 对福氏2a志贺氏菌（Shigella flexneri 2a,F2a）的志贺氏肠毒素1（Shigella enterotoxin 1，SET1）和志贺氏肠毒素2（Shigella enterotoxin 2，SET2）进行基因分型，提高菌痢暴发流行时同源性分析的水平。方法 对一起暴发F2a菌痢在现场流行病学调查的基础上，用聚合酶链反应（PCR）方法，检测43株F2a菌的志贺氏肠毒素1基因（set 1）和志贺氏肠毒素2基因（set 2），进行基因分型和同源性鉴定。 结果 43株F2a菌株分为3种基因型，即1株set 1（-）/set 2（+）；3株set 1（+）/set 2（-）；39株set 1（+）/set 2（+）。结论 本次菌痢暴发是经污染食物传播，暴发事件中夹杂着散发病例，set 1/set 2基因分型对F2a菌痢暴发流行中的同源性鉴定具有重要作用。 |
| 英文摘要: |
| Objective To identify homogenous clones of S.flexneri 2a, using gene distribution polymorphism of Shigella enterotoxin 1(set 1) and Shigella enterotoxin 2(set 2) in a Shigellosis outbreak caused by S.flexneri 2a. Methods In addition to field epidemiological investigation，43 strains of S.flexneri 2a were determined by polymerase chain reaction（PCR）. Results The 43 strains (1 from food, 1 from a carrier, 41 from patients) were divided into 3 clones(set1+/set2+ for 39 strains, set1-/set2+ for 1,set1+/set2- for 3 strains). Conclusion This outbreak was caused by S.flexneri 2a. The route of transmission was contaminated-food borne. Some sporadic cases were noticed during the outbreak. The method used in this study was satisfactory to the identification of S.flexneri 2a. |
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