文章摘要
张烨,李梓,郭俊峰,王敏,温乐英,郭元吉.中国2000~2001年流行性感冒流行概况[J].中华流行病学杂志,2003,24(1):4-8
中国2000~2001年流行性感冒流行概况
Influenza activity in China from 2000 to 2001
收稿日期:2002-08-27  出版日期:2014-09-15
DOI:
中文关键词: 流感病毒  抗原性变异  流行病学监测
英文关键词: Influenza viruses  Antigen switching  Epidemiological monitoring
基金项目:
作者单位
张烨 中国疾病预防控制中心病毒病预防控制所流感室, 北京, 100052 
李梓 中国疾病预防控制中心病毒病预防控制所流感室, 北京, 100052 
郭俊峰 中国疾病预防控制中心病毒病预防控制所流感室, 北京, 100052 
王敏 中国疾病预防控制中心病毒病预防控制所流感室, 北京, 100052 
温乐英 中国疾病预防控制中心病毒病预防控制所流感室, 北京, 100052 
郭元吉 中国疾病预防控制中心病毒病预防控制所流感室, 北京, 100052 
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中文摘要:
      目的: 了解中国 2000-2001年流行性感冒(流感)流行及抗原性变异情况。 方法: 鸡胚传代病毒用于抗原性分析; 病毒液提取RNA进行逆转录-聚合酶链反应 (RT PCR), 扩增产物纯化后测序。然后用 MegAlign(Version 1.03 )和 Editseq(Version 3.69)软件进行基因种系发生树分析。结果: 2001年流行的H1N1亚型病毒血凝素蛋白重链 (HA1)区氨基酸序列与A/上海/7/99(H1N1)相比, 在抗原决定簇D区的第190位发生了氨基酸替换; 基因种系发生树表明 2001年的H1N1亚型流感病毒存在基因特性不同的两系病毒株。国内人群中仍然同时流行着两种抗原性明显不同的B型流感病毒 (Yamagata系和Victoria系), Yamagata系病毒占大多数, Victoria系的HA1区基因与B/山东/7/97毒株相比, 其197和199位氨基酸发生了替换。 B型的基因种系发生树也证实Victoria系病毒株的抗原性改变。 2000年分离的H3N2亚型流感病毒的HA1区氨基酸序列与A /悉尼/5/97(H3N2)间有7~8个氨基酸的差异; 2001年分离的H3N2病毒株与2000年的病毒株相比, 又在 83、186、202、222位发生了氨基酸替换, 表明H3N2亚型病毒株间的抗原性发生了较明显的变异。结论: 2000-2001年中国流感的流行情况较为平静; H3N2亚型的抗原性发生?
英文摘要:
      Objective:To understand the epidemics and antigenic drift of influenza viruses in China from 2000 to 2001. Methods: The viruses were grown in embry onated hen eggs with 9-10 days old. The egg allantoic fluids with influenza viruses were used. Virion RNA was transcribed into cDNA by reverse transcriptase while cDNA amplified by PCR. Producs of PCR were purified. RNA sequence analysis was then performed. Finally, phylogenetic analysis of the sequencing data was performed with MegAlign (Version 1.03) and Editseq (Version 3.69) softw are. Results: Data from comparison of amino acid sequence on HA1 domain of HA protein molecule betw een H1N1 viruses isolated in 2001 and A Shanghai 799 (H1N1) strain indicated that there was only one difference of amino acid located at 190 position (antig enic determinant D). However, phy logenctic analy sis showed that there were two distinguishable genetically lineages of H1N1 viruses co-circulating in men in China in 2001. Two antigenically distinct genetic lineages of influenza B viruses were still existing in men in China. Most of influenza B viruses were Yamag ata-like strain and there were two different amino acid sequences located at 197 and 199 position on HA1 domain of HA protein molecule, between Victo ria-like virus isolated and B Shandong 797 strain. When comparing amino acid sequences on HA1 protein domain of H3N2 viruses isolated in 2000 with those of A Sydeney 5 97 (H3N2)virus, it was revealed that there were 7-8 differences of amino acid sequences between them. However, there were four differences related to amino acid sequences on HA1 protein domain between H3N2 viruses isolated in 2000 and in 2001. These results were fur ther demo nstra ted by analysis of pholy genic tree. Conclusions: Influenza was not prevalent in China from 2000 to 2001. The antigenic drifts of H3N2 and B Victoria-like viruses occurred. Two antigenically distinct genetic lineages of influenza B viruses were still co-circulating in men in China. Two genetically distinct lineages of influenza A (H1N1)virus were also co-circulating in men in China.
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