文章摘要
何剑峰,郑夔,黎薇,罗会明,李灵辉,毕德增,张远富,常炳功.广东省斑点热群立克次体自然疫源地调查[J].中华流行病学杂志,2003,24(8):700-703
广东省斑点热群立克次体自然疫源地调查
Study on spotted fever group Rickettsiae in Guangdong province
收稿日期:2002-08-20  出版日期:2014-09-16
DOI:
中文关键词: 斑点热立克次体;蜱;序列分析
英文关键词: Spotted fever group Rickettsia ;Tick ;Sequence analysis
基金项目:广东省医学科技研究基金资助项目 (A1999069)
作者单位
何剑峰 广东省疾病预防控制中心 广州 510300 
郑夔 广东省疾病预防控制中心 广州 510300 
黎薇 广东省疾病预防控制中心 广州 510300 
罗会明 广东省疾病预防控制中心 广州 510300 
李灵辉 广东省疾病预防控制中心 广州 510300 
毕德增 中国疾病预防控制中心传染病预防控制所 
张远富 中国疾病预防控制中心传染病预防控制所 
常炳功 中国疾病预防控制中心传染病预防控制所 
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中文摘要:
      目的 了解广东省是否存在斑点热自然疫源地。方法 用血清流行病学方法调查广东省人群、宿主动物感染状况 ;用聚合酶链反应初筛,鸡胚卵黄囊培养法直接从标本中分离立克次体 ;用血清学试验、序列测定对分离株进行鉴定。结果 检测 860份自然人群及 321份鼠类血清标本,发现健康人群平均阳性率为 3.84 %,各调查点之间的阳性率差异有显著性 (χ2 =602.39,df =8,P<0.01),山区和平原阳性率差异无显著性 (χ2=0.32,df =1,P >0.05 ) ;鼠类阳性率为4.67%,针毛鼠、白腹巨鼠、板齿鼠的阳性率分别为 11.5 9%、12.90 %和 3.13% ;在针毛鼠、白腹巨鼠、板齿鼠发现斑点热自然感染抗体是国内首次报道 ;采集鼠脾标本 321份,未分离出菌株 ;自鼠体表采集到蜱 394匹,从2只针毛鼠体表采集的蜱中分离到 2株斑点热群立克次体,命名为GDFK5 82000株、GDFK59-2000株,经血清学鉴定为西伯利亚种,对GDFK58-2000和GDFK59-2000的OmpA基因起始部位 5 33bp片段进行克隆和测序,测序结果与Genbank中其他斑点热群立克次体相应基因片段的核苷酸进行比较,GDFK58-2000、GDFK59-2000和西伯利亚立克次体之间的核苷酸同源性为 99.6 %~ 100%,推断氨基酸同源性为 100 %。结论 从宿主动
英文摘要:
      Objective To explore the existence of spotted fever group Rickettsiae (SFGR) in Guangdong province. Methods Sera were tested to find the SFGR in population and host animals. The target samples were screened by polymerase chain reation (PCR), and Rickettsiae was isolated with embryonated hen eggs and identified by serological tests. Results Eight hundred and sixty people in natural condition and 321 of mice were determined. The mean positive rate of healthy population was 3.84 %. To compare results among elected places, Fisher's exact test was applied. The difference was suggestive ( P 0.01 ), and there was no significant difference between mountain and plain areas. There was also no significant difference between mountain and plain areas ( P 0.05 ). Positive rate of mice was 4.67 %, with Rattus fulvescens, Rattus edwardsi, Bandicota indica 11.59 %, 12.90 %, 3.13 % respectively. It was the first time that SFGR antibodies in Rattus fulvescens, Rattus edwardsi, Bandicota indica were reported. A total number of 321 mice spleens and 394 ticks from the surface of mice body were collected. Two strains of SFGR, GDFK58-2000 and GDFK59-2000, were isolated in the ticks from the body surface of 2 Rattus fulvescens. They were identified as Rickettsia sibirica by serological tests. Five hundren thirty-three bp OmpA gene fragments of the two strains were cloned and sequenced. Compared with other relevant strains in Genbank, the rates of homology of nucleotide sequences of GDFK58-2000 and GDFK59-2000 and other Rickettsia sibirica strains were from 99.6 % to 100%, and the homology of amino acid speculated was 100%.Conclusion It has been proved that epidemic areas of north Asia tick-transmitted SFGR, did exist in Guangdong province confirmed by hostanimals,transmission vectors and aetiology.
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