| 许红梅,任红,卿玉玲,彭明利,凌宁.重庆地区乙型肝炎病毒B/adw2和C/adrq+亚型PreS/S基因参照序列的初步建立[J].中华流行病学杂志,2003,24(10):913-916 |
| 重庆地区乙型肝炎病毒B/adw2和C/adrq+亚型PreS/S基因参照序列的初步建立 |
| Establishment of consensus sequence of PreS S of hepatitis B virus with genotype B serotype adw2 orgenotype C serotype adrq+ prevailing in Chongqing of China |
| 收稿日期:2002-08-21 出版日期:2014-09-16 |
| DOI： |
| 中文关键词: 肝炎病毒,乙型 PreSS基因 亚型 参照序列 |
| 英文关键词: Hepatitis B virus PreS/S gene Subty pe Consensus sequence |
| 基金项目:国家自然科学重点基金资助项目( 396 30 2 80 ) |
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| 中文摘要: |
| 目的建立重庆地区不同亚型乙型肝炎病毒(HBV)流行株PreSS基因参照序列。方法扩增18例HBV慢性无症状携带者的PreSS基因,应用同源性分析和对齐比较等方法确定基因型血清型,并选同HBV亚型的PreSS拟定参照序列。结果9株基因型B血清型adw2、6株基因型C血清型adrq+、3株基因型B血清型ayw1;建立的重庆地区基因型B血清型adw2、基因型C血清型adrq+HBV流行株PreSS参照序列,与华东华南、东北华南地区同亚型的参照序列比较分别有6个、13个核苷酸差异,均可引起4个氨基酸差异。结论初步建立了重庆地区HBV基因型B血清型adw2、基因型C血清型adrq+HBV流行株PreSS基因参照序列 |
| 英文摘要: |
| Objective To develop consensus sequence of HBV PreS S with different subty pe in Chongqing of China.Methods The gene of PreS S of HBV in 18 AsC was sequenced.The genotype and sero ty pe of HBV were determined.The main HBV strain prevailing in Cho ngqing was identified to establish its co nsensus sequence.Results It was found that 9 strains were geno ty pe B serotype adw2, 6 were
g enotype C serotype adrq+ and 3 were geno ty pe B serotype ayw1.The co nsensus sequence of PreS S of HBV with genotype B serotype adw2 and g enotype C serotype adrq + were established.There were 6 nucleotide variants which causing 4 amino acids change between consensus sequence of PreS S with g enotype B serotype adw2 in Chongqing and in Southeast of China (homology showed 99.5% and 99.0 % respectiv ely).There were 13 different sites that caused 4 amino acids change between consensus sequence of PreS S with genotype C serotype adrq + in Chong qing and in No rtheast and South of China (homology showed 98.9 % and 99.0 % respectively).Comparing the two consensus sequence of PreS S gene of different subtype HBV in Chongqing, there was 95 v ariants which caused 40 amino acids variants (the homology was 92.1% and 90.0 % respectiv ely ).Conclusion The consensus sequence of PreS S of hepatitis B virus with geno type B sero ty pe adw2 and genotype C sero ty pe adrq+ prevailing in Cho ngqing was established. |
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