文章摘要
糜祖煌,秦玲.无锡地区淋病奈瑟菌TEM-1基因分子流行病学研究[J].中华流行病学杂志,2004,25(1):58-59
无锡地区淋病奈瑟菌TEM-1基因分子流行病学研究
A molecular-epidemiologic study on TEM-1 genes of Neisseria in Wuxi area
投稿时间:2002-12-25  
DOI:
中文关键词: 淋病奈瑟菌;基因;流行病学;分子
英文关键词: Neisseria gonorrhoeae;Gene;Epidemiology;molecular
基金项目:
作者单位
糜祖煌 江苏省无锡市克隆遗传技术研究所, 214026 
秦玲 江苏省无锡市克隆遗传技术研究所, 214026 
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中文摘要:
      目的 调查无锡地区淋病奈瑟菌株TEM-1基因流行情况。方法 参照国外的β;-内酰胺酶阳性的淋病奈瑟菌菌株内含TEM-1基因的质粒序列,自行设计淋病奈瑟菌TEM-1基因的半套式聚合酶链反应(heminested PCR)检测方法。并对2002年1~;10月自无锡地区分离的195株菌进行检测。结果 195株淋病奈瑟菌临床分离株中有138株为TEM-1基因阳性,阳性率为70.8%;。1株无锡地区分离株的TEM-1基因半套式PCR产物,经DNA测序与核酸库登录的β;-内酰胺酶阳性的淋病奈瑟菌质粒pFA7序列99%;同源。结论 含TEM-1基因的淋病奈瑟菌株是无锡地区的流行株。
英文摘要:
      Objective In order to study the epidemiology of TEM-1 genes of Neisseria gonorrhoeae (Ng) in Wuxi area.Methods In the light from foreign literature that pooitive Ng strains of β-lactamase contain plasmid sequences of TEM-1 genes, heminested PCR for TME-1 genes of Ng detection was self-designed.Ng of 195 strains were detected, that were isolated in Wuxi area from Jan to the Oct, 2002.Results There were 138 TEM-1 genes positive in 195 isolated strains with a positive rate of 70.8%.There was one case of heminested PCR product of TEM-1 genes which showed the homogeneity was 99%in Wuxi area, when comparing with pFA7 sequences of positive Ng plasmid of β-lactalase from register GenBank.Conclusion Data showed that the Ng strains with TEM-1 genes were the prevalent ones in Wuxi area.
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