文章摘要
张丽杰,杜曾庆,章青,康宏宇,郑丽舒,刘晓梅,谢华萍,杨红雁,王艳春,Ivanoff B,Glass RI,Bresee JS,Jiang X,Kilgore PE,方肇寅.昆明市儿童医院1998~2001年轮状病毒哨点监测分析[J].中华流行病学杂志,2004,25(5):396-399
昆明市儿童医院1998~2001年轮状病毒哨点监测分析
Rotavirus surveillance data from Kunming Children's Hospital, 1998-2001
投稿时间:2003-08-14  
DOI:
中文关键词: 轮状病毒;腹泻;哨点医院;监测;流行病学
英文关键词: Rotavirus;Diarrhea;Sentinel hospital;Surveillance;Epidemiology
基金项目:世界卫生组织课题基金(271/18/123);美国NIH课题基金(R03TW01192);国家“863”计划基金项目(2001AA21217)
作者单位E-mail
张丽杰 中国疾病预防控制中心病毒病预防控制所, 北京 100052  
杜曾庆 昆明市儿童医院  
章青 中国疾病预防控制中心病毒病预防控制所, 北京 100052  
康宏宇 昆明市儿童医院  
郑丽舒 中国疾病预防控制中心病毒病预防控制所, 北京 100052  
刘晓梅 昆明市儿童医院  
谢华萍 中国疾病预防控制中心病毒病预防控制所, 北京 100052  
杨红雁 昆明市儿童医院  
王艳春 昆明市儿童医院  
Ivanoff B Department of Vaccines and Biologicals, WHO, Geneva, Switzerland  
Glass RI Viral Gastroenteritis Section, CDC, Atlanta, GA, USA  
Bresee JS Viral Gastroenteritis Section, CDC, Atlanta, GA, USA  
Jiang X Children's Hospital Medical Center, Cincinnati, OH  
Kilgore PE International Vaccine Institute, Seoul, Korea  
方肇寅 中国疾病预防控制中心病毒病预防控制所, 北京 100052 fangzhyn@263t.net 
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中文摘要:
      目的: 了解昆明市轮状病毒腹泻的流行状况。方法: 以昆明市儿童医院为哨点监测, 监测对象为5岁以下腹泻住院患儿, 收集患儿的临床资料和粪便标本进行轮状病毒的检测和分型。病毒检测用聚丙烯酰胺凝胶电泳(PAGE)和酶联免疫吸附试验(ELISA), 毒株分型用ELISA和/或反转录-聚合酶链反应(RT-PCR)。结果: 3年监测中共收集466份腹泻患儿的粪便标本, 轮状病毒的检出率为52.8%(246/466)。轮状病毒感染97%发生于2岁以下儿童。感染有明显的季节性, 10~12月份是流行季节。对204份轮状病毒阳性标本进行G分型, G1型为流行优势株, 占47.5%, 其次为G2型(17.6%)、G3型(15.7%)G9型(4.9%)和G4型(1.0%)。P基因型以P[4]、P[8]和P[6]型为常见。最常见的P-G组合型是P[4]G2, 占34.1%(14/41), 其次是P[8]G1和P[6]G9, 分别占29.3%(12/41)和12.2%(5/41), 还有其他7种不常见的P-G组合的毒株类型。结论: 轮状病毒是昆明地区儿童腹泻住院的主要病原, 毒株呈现型的多样性, 应该开发和应用轮状病毒疫苗预防控制其流行。
英文摘要:
      Objective: To study the epidemiological status on rotavirus diarrhea in Kunming to improve the rotavirus vaccine immunization program. Methods: A hospital-based sentinel surveillance program for rotavirus was set up among children less than 5 years old with acute diarrhea in Kunming Children's Hospital. Clinical information and fecal specimens were collected and rotavirus were detected by polyacrylamide gel electrophoresis(PAGE) and/or enzyme linked immunosorbent assay(ELISA). Positive specimens were futher serotyped or genotyped by ELISA and/or RT-PCR. Results: During the three years of surveillance, 466 specimens were collected. Rotavirus were detected on 246(52.8%) specimens. 97% of the rotavirus diarrhea cases occurred among children less than 2 years old. There was a peak of admission for rotavirus diarrhea cases between October and December which accounted for 48% of all the rotavrus hospitalizations each year. Among 204 specimens with G serotyping, the predominant strain was serotype G1 (47.5%) followed by G2(17.6%), G3(15.7%), G9(4.9%) and G4(1.0%). Mixed infection(2.5%) were rate and 22 specimens(10.8%) remained non-typeable.P genotyping showed P[4]. P[8] and P[6] were the most common strains, accounting for 29.3%, 27.6% and 13.8% respectively. P[4]G2 was the most common strain which accounted for 34.1%(14/41) followed by P[8]G1(29.3%) and P[6]G9(12.2%). Another 7 uncommon P-G combinations were also identified. Conclusion: Rotaavirus was the major cause diarrhea in Kunming. An effective rotavirus vaccine for prevention and control of rotavirus diarrhea should be developed.
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