文章摘要
黄支密,毛培华,陈榆,仵蕾,吴晶.鲍曼不动杆菌SHV型β-内酰胺酶耐药基因分子流行病学研究[J].中华流行病学杂志,2004,25(5):425-427
鲍曼不动杆菌SHV型β-内酰胺酶耐药基因分子流行病学研究
Study on the molecular epidemiology of SHV type B-lactamase-encoding genes of multiple-drug-resistant acinetobacter baumannii
投稿时间:2003-05-09  
DOI:
中文关键词: 鲍曼不动杆菌;β-内酰胺酶类;基因;流行病学;分子;序列分析
英文关键词: Acinetobacter baumannii;B-lactamases'Genes;Epidemiology;molecular;Sequence analysis
基金项目:
作者单位
黄支密 解放军第九八医院检验科, 医务处, 浙江湖州 313000 
毛培华 解放军第九八医院检验科, 医务处, 浙江湖州 313000 
陈榆 解放军第九八医院检验科, 医务处, 浙江湖州 313000 
仵蕾 解放军第九八医院检验科, 医务处, 浙江湖州 313000 
吴晶 解放军第九八医院检验科, 医务处, 浙江湖州 313000 
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中文摘要:
      目的: 分析自临床分离的产SHV型β-内酰胺酶(β-lactamases, BLA)多重耐药鲍曼不动杆菌的SHV型BLA耐药基因序列并确定其所产BLA耐药基因亚型。方法: 在2000年7月至2002年12月间从临床分离60株鲍曼不动杆菌, 经药敏试验、超广谱β-内酰胺酶(FSBLs)表型确证试验和SHV型BLA耐药基因的聚合酶链反应(PCR)检测, 筛选出2株具有多重耐药特性、SHV基因型均阳性的分离株(HZ02、HZ10株), 对耐药基因进行序列分析。结果: 在2001年6月至2002年1月间分离到的18株(30.0%)鲍曼不动杆菌质粒上携带SNV型BLA耐药基因; NZ02株和NZ10株的SHVPCR扩增阳性, 其基因片段分别含825个和833个核苷酸, 与Nuesch-lnderbinen等首先发现的SHV-12亚型ESBLs编码基因序列(GenBank注册号: X98105)完全相同。结论: 30.0%鲍曼不动杆菌质粒上携带SHV型(超广谱)β-内酰胺酶耐药基因并引起一次医院感染爆发。同时证实在我国浙江省湖州地区临床分离的鲍曼不动杆菌中存在产SHV-12亚型ESBL_s耐药基因菌株。
英文摘要:
      Objective: To analyse the plasmid-mediated SHV type B-lactamases-encoding genes sequence and to identify its subtype of multiple-drug-resistant acinetobucter baumannii isolated from Huzhou district, Zhejiang province, China. Methods: Sixty strains of acineetobacter baumannii were isolated from hospitalized patients between Jul,2000 and Dec,2002. Suscepibility of antimicrobial agents and confirmatory tests for Extended-spectrum B-lactamases (ESBLs) were tested by microdilute method. SHV type B-lactamases-encooding genes were tested by polymerase chain reaction (PCR). SHV sequences of acinetobucter baunannii HZ02 and HZ10 strains were dected by ABI automated sequencer and were analysed to compare with SHV genes that had been published in GenBank. Results: Eighteen (30.0%) HZtrains of acinetobacter baumannii isolated between Jun,2001 and Jan, 2002 were carrying SHV B-lactamases resistant gene of plasmids. Detected SHV sequences of acinetobactEr baumannii HZ02 strain and HZ10 strain had 825 and 833 nucleotides respectively and had the same gene sequence as the gene encoding SHV-12 subtype of ESBLs discovered in Switzerlang. Conclusions: Thirty percentage of the clinically isolated acinetobacter banmannii were carrying SHV type (extended-spectrum) B-lactamases resistant gene of plasmids and causing an outbreak in hospital and was discovered to have carried the strains of SHV-12 subtype producing ESBLs gene in acinetobucter baumannii which was the fiest reported case in the world.
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