| 王鸣,景怀琦,徐慧芳,蒋秀高,阚飙,刘起勇,万康林,崔步云,郑翰,崔志刚,闫梅英,梁未丽,王洪霞,齐小保,李振军,李马超,陈凯,张恩民,张守印,海荣,俞东征,徐建国.广州市2004年某野生动物市场动物携带SARS-CoV监测[J].中华流行病学杂志,2005,26(2):84-87 |
| 广州市2004年某野生动物市场动物携带SARS-CoV监测 |
| Surveillance on severe acute respiratory syndrome associated coronavirus in animals at a live animal market of Guangzhou in 2004 |
| 收稿日期:2004-12-31 出版日期:2014-09-18 |
| DOI： |
| 中文关键词: 冠状病毒 严重急性呼吸综合征 果子狸 野生动物市场 |
| 英文关键词: Coron avirus seVere acute respiratory syndrome Paguma lanata Live an imal market |
| 基金项目:国家“863”高技术研究发展计划专项基金资助项目(2003AA208407) |
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| 中文摘要: |
| 目的 了解广州市某野生动物市场动物携带严重急性呼吸综合征冠状病毒 (SARS CoV)的动态变化,预测 2 0 0 4 - 2 0 0 5年冬季当地发生动物源性SARS的危险性。方法 在SARS发病相关月份采集广州市某野生动物市场动物的肛拭子和咽拭子标本,使用实时荧光聚合酶链反应 (PCR)初筛,使用RT PCR和序列分析方法核实。结果 在 2 0 0 4年 1月 5日广东省开始清除野生动物行动前采集的 31只动物 (5只赤狐、2 0只猫、6只黄毛鼠 )中,有 8只动物SARS CoV检测阳性 (2 5 .8% ),包括 3只赤狐、4只猫、1只黄毛鼠。在 2 0 0 4年 1月 2 0日采集的 119只动物 (6只兔、13只猫、4 6只原鸡、13只斑嘴鸭、10只灰雁、31只鹧鸪 )中,仅 1只灰雁的肛拭子检测阳性。 2 - 11月份采集的 10 2只动物 (14只灰雁、3只猫、5只兔、9只斑嘴鸭、2只鹧鸪、8只雉鸡、6只鸽、9只黄麂、19只山猪、16只黄毛鼠、5只犬、1只水貂、3只山羊、2只绿孔雀 )中,仅 4月份采集的 1只山猪的肛拭子标本阳性。 11- 12月份在广东省采集的 2 2只果子狸标本均为阴性。结论 和SARS流行或出现散发病例 2 0 0 3年 5月、2 0 0 4年 1月5日某野生动物市场动物的SARS CoV的RT PCR检测情况相比,2 0 0 4年 1月 2 0日至 12月广州市某野生动物市场的动物携带SARS CoV的 |
| 英文摘要: |
| Objective To study the prevalence of severe acute respiratory syndrome coronavirus (SARS CoV) like virus in animals at a live animal market of Guanzhou in 2004 before and after culling of wild animal action taken by the local authority, in order to predict the re emerging of SARS from animal originals in this region. Methods Animals at live animal market were sampled for rectal and throat swabs in triplicate. A single step realtime reverse transcription polymerase chain reaction (RT PCR) diagnostic kit was performed for screening SARS CoV like virus, the manual nested RT PCR and DNA sequencing were performed for confirmation. Only specimens which tested positive for both of the N and P genes by nested RT PCR were scored as positive. Results In 31 animals sampled in January 5 2004 before culling of wild animals at Guangdong Province,including 20 cats ( Felis catus), 5 red fox( Vulpes vulpes ) and 6 Lesser rice field rats ( Rattus losea ),8( 25.8 %) animals weretested positive for SARS CoV like virus by RT PCR methods, of which 4 cats, 3 red fox and one Lesser rice field rats were included. However, two weeks after culling of animals and disinfection of the market were implemented, in 119 animals sampled in January 20 2004, including 6 rabbits ( Oryctolagus cuniculus ),13 cats, 46 red jungle fowl ( Gallus gallus ),13 spotbill duck( Anas platyrhynchos ),10 greylag goose( Anser anser ),31 Chinese francolin( Franclinus pintadeanus ), only rectal swab from one greylag goose was tested positive for SARS CoV like virus. Furthermore, in 102 animals that including 14 greylag gooses, 3 cats, 5 rabbits, 9 spotbill duck(Anaspoecilorhyncha), 2 Chinese francolin ( Franclinus pintadeanus ), 8 common pheasant( Phasianus colchicus ), 6 pigeons, 9 Chinese muntjac( Muntiacus reevesi ),19 wild boar( Sus scrofa ),16 Lesser rice field rats, 5 dogs, 1 mink( Mustela vison ),3 goats, 2 green peafowl( Pavo muticus ) sampled in April, May, June, July, August and November, only rectal swab from one pig was tested positive. However, of 12 and 10 palm civets sampled in November and December including five of which had been at the live animals market for 2 days, none of them was tested positive. Conclusion This findings revealed that animals being sampled in April, May, June, July, August and November of 2004, only one rectal swab from a pig was tested positive as SARS CoV like virus, much lowere than the results from the previous year, suggesting that the possibility of re emerging of human infection from animal origins is low for the winter of 2004 2005. |
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