文章摘要
张潇,郑英杰,王法弟,高眉扬,朱建福,姜庆五.中国南方某农村地区人与猪戊型肝炎病毒的检测和部分序列分析[J].中华流行病学杂志,2005,26(12):984-987
中国南方某农村地区人与猪戊型肝炎病毒的检测和部分序列分析
Detection and analysis of partial sequences isolated from human and swine in rural area of southern China
投稿时间:2005-02-05  
DOI:
中文关键词: 戊型肝炎病毒;基因型;人兽共患性疾病
英文关键词: Hepatitis E virus;Genotype;Zoonosis
基金项目:复旦大学青年科学基金(JKF201001)
作者单位E-mail
张潇 中南大学公共卫生学院流行病学教研室 长沙 410078  
郑英杰 复旦大学公共卫生学院流行病学教研室 上海 200032  
王法弟 浙江省德清县疾病预防控制中心  
高眉扬 复旦大学公共卫生学院流行病学教研室 上海 200032  
朱建福 浙江省德清县疾病预防控制中心  
姜庆五 复旦大学公共卫生学院流行病学教研室 上海 200032 qwjiang@shmu.edu.cn 
摘要点击次数: 455
全文下载次数: 
中文摘要:
      目的调查中国南方某农村地区戊型肝炎病毒(HEV)人株与猪株的相关性。方法应用逆转录-巢式聚合酶链法(RT-nPCR)对一般人群中HEV-IgM阳性者、急性戊型肝炎患者和当地某养猪场的猪进行HEV RNA检测,并对HEV RNA阳性标本进行克隆测序和序列分析。结果 132份猪粪便标本中13份为HEV RNA阳性;26份lgM阳性一般人群血清标本中有4份HEV RNA阳性;4例急性戊型肝炎患者中有1例的血清和粪便标本为阳性。序列分析发现该地区HEV人株与猪株在ORE2部分区域的核苷酸序列同源性为89.3%~100.0%,这10株HEV序列与HEV Ⅰ型、Ⅱ型、Ⅲ型和Ⅳ型在同一区域的核苷酸序列的同源性分别为78.7%~84.7%、83.3%~85.3%、76.0%~80.0%和84.7%~95.3%。结论该地区人群及猪群中流行的HEV同属基因Ⅳ型。
英文摘要:
      Objective To study the genotype of swines isolated from humans and their relationships with hepatitis E virus (HEV) in the rural areas of southern China.Methods Specimens collected from normal people with HEV-IgM positive, acute hepatitis E patients and from swine in the same area were detected for HEV RNA using RT-nPCR with ORF2 primers.The positive PCR products were cloned and sequenced.Results 13 out of the 132 samples from swine stool, 4 of 26 HEV-IgM positive sera of normal people and 1 of 4 acute hepatitis E patients' stool sample and sera were tested positive for HEV RNA.Data from sequence analysis showed that the identity at nucleotide level was 89.3%-100.0% among the 10 isolates which shared 78.7% - 84.7%, 83.3%-85.3%, 76.0%-80.0% and 84.7%-95.3% nucleotide sequence identity with HEV genotype Ⅰ, Ⅱ, Ⅲ and Ⅳ respectively in the region (nt6317-6466).Conclusion HEV circulating in humans and swine in the area belonged to genotype Ⅳ.
查看全文   Html全文     查看/发表评论  下载PDF阅读器
关闭