文章摘要
张平安,吴健民,李艳.干扰素γ基因内含子1+874位点多态性与乙型肝炎病毒感染关系的研究[J].中华流行病学杂志,2006,27(1):41-43
干扰素γ基因内含子1+874位点多态性与乙型肝炎病毒感染关系的研究
Relationship between genetic polymorphisms of Interferon2gamma gene intron 1 + 874 site and susceptibilityof hepatitis B virus infection
收稿日期:2005-04-11  出版日期:2014-09-12
DOI:
中文关键词: 乙型肝炎病毒;干扰素γ基因;多态现象(遗传);易感性
英文关键词: Hepatitis B virus;Interferon-gamma gene;Polymorphism(Genetics);Susceptibility
基金项目:
作者单位
张平安 武汉大学人民医院检验科 430060 
吴健民 华中科技大学同济医学院附属协和医院检验科 
李艳 武汉大学人民医院检验科 430060 
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中文摘要:
      目的研究干扰素γ(IFN-γ)基因内含子1+874位点多态性与乙型肝炎病毒(HBV)感染、转归的关系,探讨慢性HBV感染的遗传易感因素。方法采用序列特异性引物-聚合酶链反应(PCR-SSP)技术检测231例慢性HBV携带患者、165例自限性HBV感染者和135名正常对照者IFN-γ基因内含子1+874位点T/A单核苷酸多态性。结果慢性HBV感染组IFN-γ基因+874位点AA基因型频率(TT、TA、AA频率分别为9.1%、12.1%、78.8%)高于正常对照组(TT、TA、AA频率分别为12.6%、23.0%、64.4%)(χ2=9.60,P=0.008);而自限性HBV感染组(TT、TA、AA频率分别为13.9%、23.7%、62.4%)和对照组之间差异无显著性(χ2=0.16,P=0.92)。结论IFN-γ基因内含子1+874位点多态性与慢性HBV感染有关,该位点多态性可能在决定个体HBV感染遗传易感性方面有一定意义。
英文摘要:
      ObjectiveTo explore the possible relationship between the polymorphisms of interferon-gamma ( IFN2γ) gene intron 1 at position + 874 and the susceptibility of hepatitis B virus infection. Methods IFN2γgene single nucleotide polymorphism(intron 1 at position + 874) were detected in 531 subjects including 231chronic carrier of hepatitis B virus (group CC), 165 individuals who spontaneously recovered from HBV infection(group SR) and 135 healthy controls, by polymerase chain reaction with sequence specific primers. ResultsSignificant difference of IFN2γ + 874 AA genotype frequency was found between group CC ( TT, TA and AA frequencies were 9. 1 %, 12. 1 % and 78. 8 %, respectively) and control group (TT, TA and AA frequencies were 12. 6 %, 23. 0 % and 64. 4 %, respectively) (χ2 = 9. 60, P = 0. 008), but no significant difference found between group SR(TT, TA and AA frequencies were 13. 9 %, 23. 7 % and 62. 4 %, respectively) and control group (χ2 =0. 16, P = 0. 92). Conclusion There was association between IFN2γgene polymorphism(intron 1 at position +874) and chronic HBV infection. This study suggested the possibility that IFN2γ gene polymorphism(intron 1 at position + 874) might be important in determining an individual’s susceptibility in the development of chronic HBV infection.
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