| 冯燕,严菊英,卢亦愚,史雯,茅海燕,周敏,余蓓蓓.浙江省2005年麻疹病毒流行株基因特性分析[J].中华流行病学杂志,2006,27(5):406-408 |
| 浙江省2005年麻疹病毒流行株基因特性分析 |
| Analysis on the genetic characteristics of the wide-type virus circulating in Zhejiang province in 2005 |
| 收稿日期:2005-06-09 出版日期:2014-09-12 |
| DOI： |
| 中文关键词: 麻疹病毒 基因型别 核苷酸序列 |
| 英文关键词: Measles virus Genotype Nucleic acid sequence |
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| 中文摘要: |
| 目的分析浙江省2005年麻疹病毒流行株的基因特性。方法对暴发疫情中分离的 4株麻疹病毒采用逆转录-聚合酶链反应(RT-PCR)扩增血凝素蛋白(H)和核蛋白(N)基因,纯化产物进行核苷酸序列测定。结果浙江省2005年分离到的4株麻疹病毒流行株均为麻疹H1基因型,属于中国麻疹病毒优势基因型。各分离株之间H基因氨基酸同源性为99．2％-99．7％,N基因氨基酸同源性为99．8％;与中国疫苗株沪191株的H基因和N基因比较,其氨基酸水平上的同源性分别为 95．2％-95．5％和95．5％。结论浙江省2005年分离到的麻疹病毒流行株属于同一性状毒株,均为 H1基因型;与中国疫苗株沪191株相比较,两者在基因特性上存在明显差异。 |
| 英文摘要: |
| Objective To study the genetic characteristics of measles viruses circulating in Zhejiang province in 2005. Methods 4 groups of measles viruses isolated in outbreaks and the H and N gene were amplified by reverse transcription polymerase chain reaction(RT-PCR).PCR products were purified, sequenced and data was analyzed. Results All of the 4 measles isolates belonged to genotype Hl which had been a main genotype containing all of the isolates in China. The isolates shared 99. 2%-99.7% identity of amino acid sequence on H and 99.8% identity of amino acid sequence on N gene. When comparing to the China vaccine strain ( Shanghai 191)，there were 95.2%一95. 5 % homogeneties and 95. 5 % homogenetieson H and N gene respectively. Conclusion Data from phylogenic trees of H and N gene revealed that the wide-type measles viruses circulating in Zhejiang province in 2005 all belonged to genotype H1.There were obvious differences on genetic characteristics between the isolates and the genotype A (Shanghai 191). |
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